Clonal analysis of the undifferentiated wing disk of Drosophila

Using a new cell marker, we have examined the early clonal restrictions in wing imaginal disks from late third instar larvae of Drosophila. Large clones do not significantly alter the gross structure of the disks, allowing us to map the clones relative to morphological landmarks. Clones in the posterior region of the disks behave in a similar way to clones in the adult cuticle; that is, they appear to be restricted to a defined compartment, and the presumptive anterior/posterior compartment border defined by these clones is located in a similar place in every disk. In contrast, clones in the anterior region of the wing disks often cross into the region normally occupied by the posterior compartment and, especially near the margins of the disk, show no common posterior boundary. We believe that the anterior clones are “pushing” the anterior/posterior compartment border, and that this pushing is related to the growth advantage of the marked cells, which are Minute + in a Minute background. Finally, we find that clones do not cross between the adepithelial cells, which contribute to the adult musculature, and the disk epithelium.