Isolation of genomic DNA from buccal swabs for forensic analysis, using fully automated silica-membrane purification technology

We developed a reliable and efficient, fully automated method for high-throughput isolation of genomic DNA from buccal swabs, based on silica-membrane technology. It was demonstrated that DNA purified using this method performed successfully in downstream applications relevant to forensic science an...

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Veröffentlicht in:	Legal medicine (Tokyo, Japan) Japan), 2003-03, Vol.5, p.S145-S149
Hauptverfasser:	Hanselle, Thomas, Otte, Michael, Schnibbe, Thomas, Smythe, Emma, Krieg-Schneider, Frank
Format:	Artikel
Sprache:	eng
Schlagworte:	Automated extraction DNA - isolation & purification DNA extraction DNA Fingerprinting - methods Forensic science Humans Mouth Mucosa - metabolism Polymerase Chain Reaction - methods Short tandem repeat analysis Silica membrane Silicon Dioxide Swab Tandem Repeat Sequences
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Beschreibung
Zusammenfassung:	We developed a reliable and efficient, fully automated method for high-throughput isolation of genomic DNA from buccal swabs, based on silica-membrane technology. It was demonstrated that DNA purified using this method performed successfully in downstream applications relevant to forensic science and molecular diagnostics.
ISSN:	1344-6223 1873-4162
DOI:	10.1016/S1344-6223(02)00099-8