Shape transformation and burst of giant POPC unilamellar liposomes modulated by non-ionic detergent C12E8

We studied spontaneous shape transformations and burst of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) vesicles with exogeneously added non-ionic detergent octaethylene-glycol dodecylether C(12)E(8). The addition of C(12)E(8) increased the speed of the vesicle shape transformation, so that we were able to study for the first time the complete sequence of POPC vesicle shapes starting from initial spherical vesicle with long thin tubular protrusion to final shape with invagination(s). The average mean curvature of the vesicle membrane continuously decreases during this process. The shape of the invaginations is usually spherical, however also non-spherical shapes of invaginations were observed. C(12)E(8) increases amplitudes of the fluctuations of the vesicle membrane. At higher concentrations in the membrane, C(12)E(8) induces the membrane leakage and burst of the vesicles.