Ornithine decarboxylase (EC 4.1.1.17) assay based upon the retention of putrescine by a strong cation-exchange paper

A simple and direct method for the detection of ornithine decarboxylase (EC 4.1.1.17) activity is presented. It is based upon the selective binding of putrescine to Whatman P81 paper, a strong cation-exchanger, in the presence of 0.1 m NH 3. The assay is easy to perform and has an advantage over the...

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Veröffentlicht in:	Analytical biochemistry 1981-05, Vol.113 (2), p.352-355
1. Verfasser:	Djurhuus, Rune
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals assays Carboxy-Lyases - analysis Cation Exchange Resins - metabolism Chromatography, Paper Ion Exchange Resins - metabolism ornithine decarboxylase Ornithine Decarboxylase - analysis Putrescine - metabolism Rats
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	A simple and direct method for the detection of ornithine decarboxylase (EC 4.1.1.17) activity is presented. It is based upon the selective binding of putrescine to Whatman P81 paper, a strong cation-exchanger, in the presence of 0.1 m NH 3. The assay is easy to perform and has an advantage over the more frequently used CO 2 trapping methods which can yield spurious CO 2 formation due to the action of enzymes other than ornithine decarboxylase.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(81)90088-9