Estrogen decreases biglycan mRNA expression in resistance blood vessels
University of South Dakota School of Medicine, Vermillion, South Dakota 57069 Submitted 5 September 2002 ; accepted in final form 23 June 2003 This study was designed to identify new gene targets of estrogen in the mesenteric arteries and to determine whether the soy phytoestrogens could mimic estro...
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Veröffentlicht in: | American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 2003-10, Vol.285 (4), p.754-R761 |
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Zusammenfassung: | University of South Dakota School of Medicine, Vermillion, South Dakota
57069
Submitted 5 September 2002
; accepted in final form 23 June 2003
This study was designed to identify new gene targets of estrogen in the
mesenteric arteries and to determine whether the soy phytoestrogens could
mimic estrogen effects. Ovariectomized rats were treated with estradiol,
genistein, or daidzein for 4 days. The mesenteric arteries were harvested,
total RNA was extracted, mRNA was reverse transcribed in the presence of
[33 P]dCTP, and the labeled probes were hybridized with DNA
microarrays. Analysis of the microarray data identified biglycan as a target
of estrogenic regulation. Semiquantitative RT-PCR was used to confirm and
quantitate the decrease in biglycan gene expression in response to estrogen
(-37%), genistein (-15%), and daidzein (-10%). Treatment with the pure
estrogen receptor antagonist ICI-182,780 reversed the inhibition of biglycan
gene expression. The decrease in biglycan gene expression in response to
estrogens was paralleled with a decrease in biglycan protein expression.
Biglycan protein was localized to the media of the mesenteric arteries by
immunohistochemistry. Collectively, these data suggest that biglycan is a
vascular protein regulated at the genomic level by estrogens.
DNA microarray; genistein; daidzein; mesenteric arteries
Address for reprint requests and other correspondence: K. M. Eyster, USDSM,
414 E. Clark St., Vermillion, SD 57069 (E-mail:
keyster{at}usd.edu ). |
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ISSN: | 0363-6119 1522-1490 |
DOI: | 10.1152/ajpregu.00540.2002 |