Excision Repair of UV-induced Pyrimidine Dimers in Human Skin in Vivo

The induction and loss of pyrimidine dimers in human skin in vivo was determined using UV endonuclease, alkaline sucrose sedimentation, and the fluorescent detection of nonradiolabeled DNA. The number of dimers induced following exposure of the skin to radiation emitted from a Burdick UV-800 sunlamp...

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Veröffentlicht in:	J. Invest. Dermatol.; (United States) 1981-09, Vol.77 (3), p.311-313
Hauptverfasser:	D'Ambrosio, Steven M., Slazinski, Leonard, Whetstone, James W., Lowney, Edmund
Format:	Artikel
Sprache:	eng
Schlagworte:	550200 - Biochemistry 560115 - Radiation Effects on Biochemicals- In Man- (-1987) 560151 - Radiation Effects on Animals- Man Adult AZINES BASIC BIOLOGICAL SCIENCES BIOLOGICAL RECOVERY BIOLOGICAL REPAIR BODY Centrifugation, Density Gradient CHEMICAL RADIATION EFFECTS CHEMISTRY DIMERS DNA DNA Repair - radiation effects ELECTROMAGNETIC RADIATION Endonucleases - metabolism ENZYMES ESTERASES Female HETEROCYCLIC COMPOUNDS Humans HYDROLASES IN VIVO Male NUCLEASES NUCLEIC ACIDS ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS ORGANS PHOSPHODIESTERASES Pyrimidine Dimers - metabolism Pyrimidine Dimers - radiation effects PYRIMIDINES RADIATION CHEMISTRY RADIATION EFFECTS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIATIONS RECOVERY REPAIR SKIN Skin - metabolism Skin - radiation effects ULTRAVIOLET RADIATION Ultraviolet Rays
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Zusammenfassung:	The induction and loss of pyrimidine dimers in human skin in vivo was determined using UV endonuclease, alkaline sucrose sedimentation, and the fluorescent detection of nonradiolabeled DNA. The number of dimers induced following exposure of the skin to radiation emitted from a Burdick UV-800 sunlamp was quantitated by reacting the extracted DNA with Micrococcus luteus endonuclease specific for pyrimidine dimers. Exposure to 15 and 30 seconds of radiation emitted from this lamp produced the formation of 12.8 and 23.6 dimers per 108 daltons DNA, respectively. Approximately 50% of the dimers induced were lost 58min after irradiation. Only a small percentage (
ISSN:	0022-202X 1523-1747
DOI:	10.1111/1523-1747.ep12482484