Single-Molecule Kinetics of λ Exonuclease Reveal Base Dependence and Dynamic Disorder

We used a multiplexed approach based on flow-stretched DNA to monitor the enzymatic digestion of λ-phage DNA by individual bacteriophage λ exonuclease molecules. Statistical analyses of multiple single-molecule trajectories observed simultaneously reveal that the catalytic rate is dependent on the l...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2003-08, Vol.301 (5637), p.1235-1238
Hauptverfasser:	van Oijen, Antoine M., Blainey, Paul C., Crampton, Donald J., Richardson, Charles C., Ellenberger, Tom, Xie, X. Sunney
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Bacteriophage lambda - enzymology Base Composition Base Sequence Binding Sites Biological and medical sciences Catalysis Chemical properties DNA DNA, Single-Stranded - chemistry DNA, Single-Stranded - metabolism DNA, Viral - chemistry DNA, Viral - metabolism Enzymes Enzymes and enzyme inhibitors Exodeoxyribonucleases - chemistry Exodeoxyribonucleases - metabolism Exonucleases Free energy Fundamental and applied biological sciences. Psychology Genetic variation Hydrogen Bonding Hydrolysis Kinetics Melting Miscellaneous Molecules Nucleic Acid Conformation Nucleotides Protein Conformation Thermodynamics Trajectories Viral Proteins
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