Selenium in bovine spermatozoa

Selenium in bovine spermatozoa

This study investigated the association of selenium with ejaculated bovine spermatozoa. Over 75% of the radioactive spermatozoa. Over 75% of the radioactive selenium-75 was released after 30 min of incubation in 2 X 10(-3) dithiothreitol. Of the selenium-75 released by dithiothreitol, 85% was associ...

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Veröffentlicht in:Journal of dairy science 1981-05, Vol.64 (5), p.853-856
Hauptverfasser: Niemi, S. M, Kuzan, F. B, Senger, P. L
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cattle - physiology
Male
Selenium - analysis
Spermatozoa - analysis
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Zusammenfassung:This study investigated the association of selenium with ejaculated bovine spermatozoa. Over 75% of the radioactive spermatozoa. Over 75% of the radioactive selenium-75 was released after 30 min of incubation in 2 X 10(-3) dithiothreitol. Of the selenium-75 released by dithiothreitol, 85% was associated with spermatozoal protein. Protein containing selenium-75 was found predominantly in a single band after polyacrylamide gel electrophoresis. Molecular weight was approximately 21,500 daltons.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(81)82658-6