Heterogeneity of human chromosome 9 constitutive heterochromatin as revealed by sequential distamycin A/DAPI staining and C-banding

Heterogeneity of human chromosome 9 constitutive heterochromatin as revealed by sequential distamycin A/DAPI staining and C-banding

Distamycin A/DAPI staining and sequential C-banding of human lymphocyte chromosomes reveals the regular occurrence of differentially staining subfractions of chromosome 9 constitutive heterochromatin. These subfractions are regionally organized as two subsegments: a distal one, which fluoresces brig...

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Veröffentlicht in:Human genetics 1981-03, Vol.57 (1), p.28-30
Hauptverfasser: Buys, C H, Gouw, W L, Blenkers, J A, van Dalen, C H
Format: Artikel
Sprache:eng
Schlagworte:
Amidines
Chromosome Banding
Chromosomes, Human, 6-12 and X - ultrastructure
Distamycins
Fluorescent Dyes
Heterochromatin - ultrastructure
Humans
Indoles
Lymphocytes - cytology
Metaphase
Staining and Labeling
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Zusammenfassung:Distamycin A/DAPI staining and sequential C-banding of human lymphocyte chromosomes reveals the regular occurrence of differentially staining subfractions of chromosome 9 constitutive heterochromatin. These subfractions are regionally organized as two subsegments: a distal one, which fluoresces brightly with DAPI after preincubation with distamycin A and a proximal one, which stains intensely with Giemsa after sequential C-banding. Observations are presented that indicate an occasionally independent genetic behavior of these heterochromatin subfractions.
ISSN:0340-6717
1432-1203
DOI:10.1007/BF00271162