Modulation of calcium-activated potassium small conductance (SK) current in rat dopamine neurons of the ventral tegmental area

Block of calcium-sensitive potassium SK current (gKCa [SK]) by apamin or bicuculline methiodide potentiates burst firing in dopamine neurons in the presence of N-methyl- d-aspartate (NMDA). The purpose of this study was to test the hypothesis that calcium entry through NMDA-gated channels can potent...

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Veröffentlicht in:Neuroscience letters 2003-09, Vol.348 (3), p.180-184
Hauptverfasser: Paul, Kush, Keith, Dove J., Johnson, Steven W.
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Sprache:eng
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Zusammenfassung:Block of calcium-sensitive potassium SK current (gKCa [SK]) by apamin or bicuculline methiodide potentiates burst firing in dopamine neurons in the presence of N-methyl- d-aspartate (NMDA). The purpose of this study was to test the hypothesis that calcium entry through NMDA-gated channels can potentiate gKCa [SK] in dopamine neurons in the ventral tegmental area. We used microelectrodes to record an outward tail current that was evoked by membrane depolarization under single-electrode voltage-clamp. Using bicuculline methiodide (50 μM) as a reversible inhibitor of gKCa [SK], we found that NMDA (15 μM) reduced the peak amplitude of the outward tail current by 39%. Contrary to expectations, our results suggest that stimulation of NMDA receptors reduces the calcium-activated potassium gKCa [SK], an effect that could facilitate NMDA-dependent burst firing.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(03)00747-X