An improved method for the detection of mutants at the waxy locus in hordeum vulgare
Immature barley spikes from greenhouse and field-grown plants were harvested 2-4 days prior to anthesis and prepared for analysis. The improved procedure described permits routine and accurate scoring of large numbers of pollen grains and detection of low frequency forward or reverse mutations at th...
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| Veröffentlicht in: | Environ. Mutagen.; (United States) 1981, Vol.3 (1), p.91-93 |
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| container_title | Environ. Mutagen.; (United States) |
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| creator | Rosichan, J. L. Arenaz, P. Blake, N. Hodgdon, A. Kleinhofs, A. Nilan, R. A. |
| description | Immature barley spikes from greenhouse and field-grown plants were harvested 2-4 days prior to anthesis and prepared for analysis. The improved procedure described permits routine and accurate scoring of large numbers of pollen grains and detection of low frequency forward or reverse mutations at the waxy locus. Reversion frequencies are presented in table form for azide- and gamma-induced mutants. (JMT) |
| doi_str_mv | 10.1002/em.2860030110 |
| format | Article |
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L. ; Arenaz, P. ; Blake, N. ; Hodgdon, A. ; Kleinhofs, A. ; Nilan, R. A.</creator><creatorcontrib>Rosichan, J. L. ; Arenaz, P. ; Blake, N. ; Hodgdon, A. ; Kleinhofs, A. ; Nilan, R. A. ; Washington State Univ., Pullman</creatorcontrib><description>Immature barley spikes from greenhouse and field-grown plants were harvested 2-4 days prior to anthesis and prepared for analysis. The improved procedure described permits routine and accurate scoring of large numbers of pollen grains and detection of low frequency forward or reverse mutations at the waxy locus. Reversion frequencies are presented in table form for azide- and gamma-induced mutants. 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MAT ; RADIATIONS</subject><ispartof>Environ. 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L.</creatorcontrib><creatorcontrib>Arenaz, P.</creatorcontrib><creatorcontrib>Blake, N.</creatorcontrib><creatorcontrib>Hodgdon, A.</creatorcontrib><creatorcontrib>Kleinhofs, A.</creatorcontrib><creatorcontrib>Nilan, R. A.</creatorcontrib><creatorcontrib>Washington State Univ., Pullman</creatorcontrib><title>An improved method for the detection of mutants at the waxy locus in hordeum vulgare</title><title>Environ. Mutagen.; (United States)</title><addtitle>Environ. mutagen</addtitle><description>Immature barley spikes from greenhouse and field-grown plants were harvested 2-4 days prior to anthesis and prepared for analysis. The improved procedure described permits routine and accurate scoring of large numbers of pollen grains and detection of low frequency forward or reverse mutations at the waxy locus. Reversion frequencies are presented in table form for azide- and gamma-induced mutants. (JMT)</description><subject>560141 - Radiation Effects on Plants- Basic Studies- (-1987)</subject><subject>560303 - Chemicals Metabolism & Toxicology- Plants- (-1987)</subject><subject>AZIDO COMPOUNDS</subject><subject>BARLEY</subject><subject>BIOLOGICAL EFFECTS</subject><subject>BIOLOGICAL RADIATION EFFECTS</subject><subject>CEREALS</subject><subject>Edible Grain - genetics</subject><subject>ELECTROMAGNETIC RADIATION</subject><subject>Filtration</subject><subject>GAMETES</subject><subject>GAMMA RADIATION</subject><subject>GENES</subject><subject>GENETIC EFFECTS</subject><subject>GENETIC RADIATION EFFECTS</subject><subject>GERM CELLS</subject><subject>GRAMINEAE</subject><subject>GRASS</subject><subject>Hordeum - genetics</subject><subject>IONIZING RADIATIONS</subject><subject>MEASURING METHODS</subject><subject>MUTANTS</subject><subject>Mutation - drug effects</subject><subject>MUTATION FREQUENCY</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANIC NITROGEN COMPOUNDS</subject><subject>PLANTS</subject><subject>POLLEN</subject><subject>RADIATION EFFECTS</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIATIONS</subject><issn>0192-2521</issn><issn>1930-238X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFvEzEQhS0EKiH0yBHJ4sBty9jO2rvHtiotUhpUqYjeLMeeJYb1urW9bfPvWZqoiAun0eh98_TmEfKOwRED4J8wHPFGAghgDF6QGWsFVFw0Ny_JDFjLK15z9pq8yfknQL3gsj0gB4pLPi0zcn08UB9uU7xHRwOWTXS0i4mWDVKHBW3xcaCxo2EsZiiZmvKkPZjHLe2jHTP1A93E5HAM9H7sf5iEb8mrzvQZD_dzTr59Prs-vaiWX8-_nB4vK7sABpWwUiq3kApbwaypG2NaCfXaGKYY8k5JKWrB3bqRvO3qrl00Srat41YBOiPEnHzY-cZcvM7WT3k3Ng7DFFtLzkEwOUEfd9D05N2Iuejgs8W-NwPGMWsl6kbxqb85qXagTTHnhJ2-TT6YtNUM9J-qNQb9t-qJf783HtcB3TO973bS1U5_8D1u_2-mzy7_cd4n8bng4_OlSb-0VELV-vvqXF9dXK2WqxPQJ-I3HamW_w</recordid><startdate>1981</startdate><enddate>1981</enddate><creator>Rosichan, J. L.</creator><creator>Arenaz, P.</creator><creator>Blake, N.</creator><creator>Hodgdon, A.</creator><creator>Kleinhofs, A.</creator><creator>Nilan, R. A.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>1981</creationdate><title>An improved method for the detection of mutants at the waxy locus in hordeum vulgare</title><author>Rosichan, J. L. ; Arenaz, P. ; Blake, N. ; Hodgdon, A. ; Kleinhofs, A. ; Nilan, R. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4010-3c667d467e931ca58aa9605baa171e2f7663532db8629f5f9487699d2c70eda33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>560141 - Radiation Effects on Plants- Basic Studies- (-1987)</topic><topic>560303 - Chemicals Metabolism & Toxicology- Plants- (-1987)</topic><topic>AZIDO COMPOUNDS</topic><topic>BARLEY</topic><topic>BIOLOGICAL EFFECTS</topic><topic>BIOLOGICAL RADIATION EFFECTS</topic><topic>CEREALS</topic><topic>Edible Grain - genetics</topic><topic>ELECTROMAGNETIC RADIATION</topic><topic>Filtration</topic><topic>GAMETES</topic><topic>GAMMA RADIATION</topic><topic>GENES</topic><topic>GENETIC EFFECTS</topic><topic>GENETIC RADIATION EFFECTS</topic><topic>GERM CELLS</topic><topic>GRAMINEAE</topic><topic>GRASS</topic><topic>Hordeum - genetics</topic><topic>IONIZING RADIATIONS</topic><topic>MEASURING METHODS</topic><topic>MUTANTS</topic><topic>Mutation - drug effects</topic><topic>MUTATION FREQUENCY</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANIC NITROGEN COMPOUNDS</topic><topic>PLANTS</topic><topic>POLLEN</topic><topic>RADIATION EFFECTS</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RADIATIONS</topic><toplevel>online_resources</toplevel><creatorcontrib>Rosichan, J. L.</creatorcontrib><creatorcontrib>Arenaz, P.</creatorcontrib><creatorcontrib>Blake, N.</creatorcontrib><creatorcontrib>Hodgdon, A.</creatorcontrib><creatorcontrib>Kleinhofs, A.</creatorcontrib><creatorcontrib>Nilan, R. A.</creatorcontrib><creatorcontrib>Washington State Univ., Pullman</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Environ. Mutagen.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rosichan, J. L.</au><au>Arenaz, P.</au><au>Blake, N.</au><au>Hodgdon, A.</au><au>Kleinhofs, A.</au><au>Nilan, R. A.</au><aucorp>Washington State Univ., Pullman</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An improved method for the detection of mutants at the waxy locus in hordeum vulgare</atitle><jtitle>Environ. Mutagen.; (United States)</jtitle><addtitle>Environ. mutagen</addtitle><date>1981</date><risdate>1981</risdate><volume>3</volume><issue>1</issue><spage>91</spage><epage>93</epage><pages>91-93</pages><issn>0192-2521</issn><eissn>1930-238X</eissn><abstract>Immature barley spikes from greenhouse and field-grown plants were harvested 2-4 days prior to anthesis and prepared for analysis. The improved procedure described permits routine and accurate scoring of large numbers of pollen grains and detection of low frequency forward or reverse mutations at the waxy locus. Reversion frequencies are presented in table form for azide- and gamma-induced mutants. 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| fulltext | fulltext |
| identifier | ISSN: 0192-2521 |
| ispartof | Environ. Mutagen.; (United States), 1981, Vol.3 (1), p.91-93 |
| issn | 0192-2521 1930-238X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73587200 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | 560141 - Radiation Effects on Plants- Basic Studies- (-1987) 560303 - Chemicals Metabolism & Toxicology- Plants- (-1987) AZIDO COMPOUNDS BARLEY BIOLOGICAL EFFECTS BIOLOGICAL RADIATION EFFECTS CEREALS Edible Grain - genetics ELECTROMAGNETIC RADIATION Filtration GAMETES GAMMA RADIATION GENES GENETIC EFFECTS GENETIC RADIATION EFFECTS GERM CELLS GRAMINEAE GRASS Hordeum - genetics IONIZING RADIATIONS MEASURING METHODS MUTANTS Mutation - drug effects MUTATION FREQUENCY ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS PLANTS POLLEN RADIATION EFFECTS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIATIONS |
| title | An improved method for the detection of mutants at the waxy locus in hordeum vulgare |
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