An improved method for the detection of mutants at the waxy locus in hordeum vulgare

Immature barley spikes from greenhouse and field-grown plants were harvested 2-4 days prior to anthesis and prepared for analysis. The improved procedure described permits routine and accurate scoring of large numbers of pollen grains and detection of low frequency forward or reverse mutations at th...

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Veröffentlicht in:	Environ. Mutagen.; (United States) 1981, Vol.3 (1), p.91-93
Hauptverfasser:	Rosichan, J. L., Arenaz, P., Blake, N., Hodgdon, A., Kleinhofs, A., Nilan, R. A.
Format:	Artikel
Sprache:	eng
Schlagworte:	560141 - Radiation Effects on Plants- Basic Studies- (-1987) 560303 - Chemicals Metabolism & Toxicology- Plants- (-1987) AZIDO COMPOUNDS BARLEY BIOLOGICAL EFFECTS BIOLOGICAL RADIATION EFFECTS CEREALS Edible Grain - genetics ELECTROMAGNETIC RADIATION Filtration GAMETES GAMMA RADIATION GENES GENETIC EFFECTS GENETIC RADIATION EFFECTS GERM CELLS GRAMINEAE GRASS Hordeum - genetics IONIZING RADIATIONS MEASURING METHODS MUTANTS Mutation - drug effects MUTATION FREQUENCY ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS PLANTS POLLEN RADIATION EFFECTS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIATIONS
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Beschreibung
Zusammenfassung:	Immature barley spikes from greenhouse and field-grown plants were harvested 2-4 days prior to anthesis and prepared for analysis. The improved procedure described permits routine and accurate scoring of large numbers of pollen grains and detection of low frequency forward or reverse mutations at the waxy locus. Reversion frequencies are presented in table form for azide- and gamma-induced mutants. (JMT)
ISSN:	0192-2521 1930-238X
DOI:	10.1002/em.2860030110