Direct determination of single-to-double stranded DNA ratio in solution using steady-state fluorescence measurements

Direct determination of single-to-double stranded DNA ratio in solution using steady-state fluorescence measurements

We report a simple and rapid method for quantitation of single-to-double stranded (ss : ds) DNA ratios in solution, using steady-state measurements of fluorescence from two simultaneously excited intercalated dyes; the ratio of fluorescence intensities from PicoGreen (525 nm) and ethidium bromide (6...

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Veröffentlicht in:Organic & biomolecular chemistry 2003-02, Vol.1 (3), p.450-451
Hauptverfasser: Beach, Lori, Schweitzer, Claude, Scaiano, J C
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cattle
DNA - analysis
DNA, Single-Stranded - analysis
Ethidium - analysis
Ethidium - chemistry
Fluorescent Dyes - analysis
Fluorescent Dyes - chemistry
Organic Chemicals
Solutions - chemistry
Spectrometry, Fluorescence - methods
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Beschreibung
Zusammenfassung:We report a simple and rapid method for quantitation of single-to-double stranded (ss : ds) DNA ratios in solution, using steady-state measurements of fluorescence from two simultaneously excited intercalated dyes; the ratio of fluorescence intensities from PicoGreen (525 nm) and ethidium bromide (610 nm) is directly proportional to the ss : ds DNA ratio.
ISSN:1477-0520
1477-0539
DOI:10.1039/b209284k