Single-Molecule Covalent Chemistry with Spatially Separated Reactants

In a recent study, the reversible covalent chemistry of a simple reaction was monitored at the single‐molecule level by observing the current flowing through a protein pore, which acted as a nanoreactor. Now, a multistep reaction has been observed by an improved approach, in which two of the reactants are separated by the membrane that contains the pore and diffuse into the reaction zone (see picture).