Simultaneous Determination of Hydroxyzine Hydrochloride and Benzyl Alcohol in Injection Solutions by High-Performance Liquid Chromatography

A stability-indicating, high-performance liquid chromatographic method was developed for the simultaneous determination of hydroxyzine hydrochloride and benzyl alcohol in injection solutions. Separation was achieved using a μ Bondapak C18 column and the eluent [60% water, 25% acetonitrile, and 15% methanol containing 0.06% (v/v) sulfuric acid, 0.5% (w/v) sodium sulfate, and 0.02% (w/v) heptanesulfonic acid sodium salt] at a flow rate of 2ml/min. Isobutyrophenone and p-nitroacetophenone were used as internal standards. The UV detector response at 257nm was linear for hydroxyzine hydrochloride in the 3-10-mg/ml range and for benzyl alcohol in the 0.54-1.8-mg/ml range under analysis conditions. The method is accurate, simple, and precise.