Isolation and genetic characterization of T1-transducing mutants with increased transduction frequency

Isolation and genetic characterization of T1-transducing mutants with increased transduction frequency

Seven T1 mutants that transduce at higher than wild-type efficiencies were isolated. The mutants tranduced a λ imm 434 dg prophage 50 to 27,000 times more efficiently than wild type. The mutations are located in a small region of the T1 chromosome coding for early functions between the am5 (gene 2)...

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Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 1981-01, Vol.112 (2), p.662-669
Hauptverfasser: Roberts, Mary Denton, Drexler, Henry
Format: Artikel
Sprache:eng
Schlagworte:
Genes, Viral
Genetic Linkage
Mutation
Recombination, Genetic
T-Phages - genetics
T-Phages - isolation & purification
Transduction, Genetic
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Zusammenfassung:Seven T1 mutants that transduce at higher than wild-type efficiencies were isolated. The mutants tranduced a λ imm 434 dg prophage 50 to 27,000 times more efficiently than wild type. The mutations are located in a small region of the T1 chromosome coding for early functions between the am5 (gene 2) and am6 (gene 3) mutations. The mutations are nonidentical but closely linked. Using four-factor, reciprocal crosses it was possible to determine the order of the markers.
ISSN:0042-6822
1096-0341
DOI:10.1016/0042-6822(81)90311-1