Mixed agglutination on solid phase (MASP) and its inhibition (IMASP): Two immunoassays for soluble ABH group-specific substances

Mixed agglutination on solid phase (MASP) and its inhibition (IMASP): Two immunoassays for soluble ABH group-specific substances

Two immunoassays are described: mixed agglutination on solid phase (MASP) and inhibition of mixed agglutination on solid phase (IMASP). The MASP assay permits detection and measurement of soluble A, B and H high molecular weight glycoproteins in secretor individuals with a sensitivity at least 800 t...

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Veröffentlicht in:Journal of immunological methods 1981, Vol.44 (1), p.115-123
Hauptverfasser: Fiori, Angelo, Chiarotti, Marcello
Format: Artikel
Sprache:eng
Schlagworte:
ABO Blood-Group System
Agglutination Tests
Cell Adhesion
Humans
Immune Sera - pharmacology
Immunoassay
Incubators
Saliva - immunology
Solubility
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Zusammenfassung:Two immunoassays are described: mixed agglutination on solid phase (MASP) and inhibition of mixed agglutination on solid phase (IMASP). The MASP assay permits detection and measurement of soluble A, B and H high molecular weight glycoproteins in secretor individuals with a sensitivity at least 800 times greater than the usual inhibition technique. Both secretors and non-secretors can be typed by the less sensitive IMASP test which also permits the detection of low molecular weight A, B and H substances.
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(81)90112-5