Modified single radial haemolysis technique: Measurement of antibodies to newcastle disease virus
The single radial haemolysis technique has been improved by including a central trough for complement in the agarose plate containing sensitised chick erythrocytes. The advantages are that the complement diffuses with the serum samples, significantly reducting the time required, thus minimising auto...
Gespeichert in:
| Veröffentlicht in: | Journal of immunological methods 1981, Vol.40 (2), p.239-242 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 242 |
|---|---|
| container_issue | 2 |
| container_start_page | 239 |
| container_title | Journal of immunological methods |
| container_volume | 40 |
| creator | Charan, Shiv Rai, Arvind Mahajan, V.M. Agarwal, L.P. |
| description | The single radial haemolysis technique has been improved by including a central trough for complement in the agarose plate containing sensitised chick erythrocytes. The advantages are that the complement diffuses with the serum samples, significantly reducting the time required, thus minimising autohaemolysis and increasing storage ability. In addition, the amount of complement required is much reduced.
The application of this modification to detection of antibodies against Newcastle Disease virus is described. |
| doi_str_mv | 10.1016/0022-1759(81)90070-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73545037</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0022175981900703</els_id><sourcerecordid>73545037</sourcerecordid><originalsourceid>FETCH-LOGICAL-c296t-61957245cbdc3d96931f01281e0bd337042e20bf4748768a0a905589971f3e83</originalsourceid><addsrcrecordid>eNp9kM1u1TAQRi0EKpfCG4DwCsEiMLaT2GaBhCr-pFYsKGvLsSetURIXT1LUt8eXe9Ulq1nM-T7NHMaeC3grQPTvAKRshO7sayPeWAANjXrAdsJo2WgL3UO2u0cesydEvwBAQA8n7KTXxphO7Ji_yDGNCSOntFxNyIuPyU_82uOcpztKxFcM10v6veF7foGetoIzLivPI_fLmoaaxwplvuCf4GmtHTFRBZHfprLRU_Zo9BPhs-M8ZZefP12efW3Ov3_5dvbxvAnS9mvTC9tp2XZhiEFF21slRhDSCIQhKqWhlShhGFvdGt0bD76-2BlrtRgVGnXKXh1qb0qut9Lq5kQBp8kvmDdyWnVtB0pXsD2AoWSigqO7KWn25c4JcHuxbm_N7a05I9w_sU7V2Itj_zbMGO9DR5N1__KwH312_qokcj9_SBCqPmF7oWQlPhwIrBZuExZHIeESMKaCYXUxp_-f8BcF9ZBh</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73545037</pqid></control><display><type>article</type><title>Modified single radial haemolysis technique: Measurement of antibodies to newcastle disease virus</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Charan, Shiv ; Rai, Arvind ; Mahajan, V.M. ; Agarwal, L.P.</creator><creatorcontrib>Charan, Shiv ; Rai, Arvind ; Mahajan, V.M. ; Agarwal, L.P.</creatorcontrib><description>The single radial haemolysis technique has been improved by including a central trough for complement in the agarose plate containing sensitised chick erythrocytes. The advantages are that the complement diffuses with the serum samples, significantly reducting the time required, thus minimising autohaemolysis and increasing storage ability. In addition, the amount of complement required is much reduced.
The application of this modification to detection of antibodies against Newcastle Disease virus is described.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/0022-1759(81)90070-3</identifier><identifier>PMID: 6788851</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>animal diseases ; animal health ; Animals ; Antibodies, Viral - analysis ; Chickens - blood ; Hemolytic Plaque Technique ; Immunodiffusion ; Newcastle disease virus - immunology ; viral diseases of animals and humans</subject><ispartof>Journal of immunological methods, 1981, Vol.40 (2), p.239-242</ispartof><rights>1981</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c296t-61957245cbdc3d96931f01281e0bd337042e20bf4748768a0a905589971f3e83</citedby><cites>FETCH-LOGICAL-c296t-61957245cbdc3d96931f01281e0bd337042e20bf4748768a0a905589971f3e83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-1759(81)90070-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,4022,27922,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6788851$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Charan, Shiv</creatorcontrib><creatorcontrib>Rai, Arvind</creatorcontrib><creatorcontrib>Mahajan, V.M.</creatorcontrib><creatorcontrib>Agarwal, L.P.</creatorcontrib><title>Modified single radial haemolysis technique: Measurement of antibodies to newcastle disease virus</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>The single radial haemolysis technique has been improved by including a central trough for complement in the agarose plate containing sensitised chick erythrocytes. The advantages are that the complement diffuses with the serum samples, significantly reducting the time required, thus minimising autohaemolysis and increasing storage ability. In addition, the amount of complement required is much reduced.
The application of this modification to detection of antibodies against Newcastle Disease virus is described.</description><subject>animal diseases</subject><subject>animal health</subject><subject>Animals</subject><subject>Antibodies, Viral - analysis</subject><subject>Chickens - blood</subject><subject>Hemolytic Plaque Technique</subject><subject>Immunodiffusion</subject><subject>Newcastle disease virus - immunology</subject><subject>viral diseases of animals and humans</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1u1TAQRi0EKpfCG4DwCsEiMLaT2GaBhCr-pFYsKGvLsSetURIXT1LUt8eXe9Ulq1nM-T7NHMaeC3grQPTvAKRshO7sayPeWAANjXrAdsJo2WgL3UO2u0cesydEvwBAQA8n7KTXxphO7Ji_yDGNCSOntFxNyIuPyU_82uOcpztKxFcM10v6veF7foGetoIzLivPI_fLmoaaxwplvuCf4GmtHTFRBZHfprLRU_Zo9BPhs-M8ZZefP12efW3Ov3_5dvbxvAnS9mvTC9tp2XZhiEFF21slRhDSCIQhKqWhlShhGFvdGt0bD76-2BlrtRgVGnXKXh1qb0qut9Lq5kQBp8kvmDdyWnVtB0pXsD2AoWSigqO7KWn25c4JcHuxbm_N7a05I9w_sU7V2Itj_zbMGO9DR5N1__KwH312_qokcj9_SBCqPmF7oWQlPhwIrBZuExZHIeESMKaCYXUxp_-f8BcF9ZBh</recordid><startdate>1981</startdate><enddate>1981</enddate><creator>Charan, Shiv</creator><creator>Rai, Arvind</creator><creator>Mahajan, V.M.</creator><creator>Agarwal, L.P.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1981</creationdate><title>Modified single radial haemolysis technique: Measurement of antibodies to newcastle disease virus</title><author>Charan, Shiv ; Rai, Arvind ; Mahajan, V.M. ; Agarwal, L.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c296t-61957245cbdc3d96931f01281e0bd337042e20bf4748768a0a905589971f3e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>animal diseases</topic><topic>animal health</topic><topic>Animals</topic><topic>Antibodies, Viral - analysis</topic><topic>Chickens - blood</topic><topic>Hemolytic Plaque Technique</topic><topic>Immunodiffusion</topic><topic>Newcastle disease virus - immunology</topic><topic>viral diseases of animals and humans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Charan, Shiv</creatorcontrib><creatorcontrib>Rai, Arvind</creatorcontrib><creatorcontrib>Mahajan, V.M.</creatorcontrib><creatorcontrib>Agarwal, L.P.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Charan, Shiv</au><au>Rai, Arvind</au><au>Mahajan, V.M.</au><au>Agarwal, L.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modified single radial haemolysis technique: Measurement of antibodies to newcastle disease virus</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1981</date><risdate>1981</risdate><volume>40</volume><issue>2</issue><spage>239</spage><epage>242</epage><pages>239-242</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><abstract>The single radial haemolysis technique has been improved by including a central trough for complement in the agarose plate containing sensitised chick erythrocytes. The advantages are that the complement diffuses with the serum samples, significantly reducting the time required, thus minimising autohaemolysis and increasing storage ability. In addition, the amount of complement required is much reduced.
The application of this modification to detection of antibodies against Newcastle Disease virus is described.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>6788851</pmid><doi>10.1016/0022-1759(81)90070-3</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1759 |
| ispartof | Journal of immunological methods, 1981, Vol.40 (2), p.239-242 |
| issn | 0022-1759 1872-7905 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73545037 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | animal diseases animal health Animals Antibodies, Viral - analysis Chickens - blood Hemolytic Plaque Technique Immunodiffusion Newcastle disease virus - immunology viral diseases of animals and humans |
| title | Modified single radial haemolysis technique: Measurement of antibodies to newcastle disease virus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T00%3A55%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Modified%20single%20radial%20haemolysis%20technique:%20Measurement%20of%20antibodies%20to%20newcastle%20disease%20virus&rft.jtitle=Journal%20of%20immunological%20methods&rft.au=Charan,%20Shiv&rft.date=1981&rft.volume=40&rft.issue=2&rft.spage=239&rft.epage=242&rft.pages=239-242&rft.issn=0022-1759&rft.eissn=1872-7905&rft_id=info:doi/10.1016/0022-1759(81)90070-3&rft_dat=%3Cproquest_cross%3E73545037%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73545037&rft_id=info:pmid/6788851&rft_els_id=0022175981900703&rfr_iscdi=true |