Sulfur as the Key Element for Quantitative Protein Analysis by Capillary Liquid Chromatography Coupled to Element Mass Spectrometry

Sulfur as the Key Element for Quantitative Protein Analysis by Capillary Liquid Chromatography Coupled to Element Mass Spectrometry

The impact of 32S quantification by capillary chromatography coupled to element mass spectrometry was demonstrated for three areas in proteomic analysis: for absolute quantification of a peptide, for the characterization of a protein digest (see chromatogram), and for the estimation of the efficienc...

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Veröffentlicht in:Angewandte Chemie International Edition 2003-07, Vol.42 (29), p.3425-3427
Hauptverfasser: Wind, Mathias, Wegener, Ansgar, Eisenmenger, Andreas, Kellner, Roland, Lehmann, Wolf D.
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
analytical chemistry
Chromatography, Liquid - methods
electrospray ionization
Insulin - analysis
Insulin - chemistry
mass spectrometry
Mass Spectrometry - methods
Molecular Sequence Data
Peptide Fragments - analysis
peptides
Phosphates - chemistry
Phosphorylation
proteins
Proteins - analysis
Proteins - chemistry
Spectrometry, Mass, Electrospray Ionization - methods
Sulfur - chemistry
Thiamine - analysis
Thiamine - chemistry
Time Factors
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Beschreibung
Zusammenfassung:The impact of 32S quantification by capillary chromatography coupled to element mass spectrometry was demonstrated for three areas in proteomic analysis: for absolute quantification of a peptide, for the characterization of a protein digest (see chromatogram), and for the estimation of the efficiency of electrospray ionization.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200250547