Glucosides from Vitex agnus-castus

The methanolic extract of the flowering stems of Vitex agnus-castus yielded three new iridoids: 6′- O-foliamenthoylmussaenosidic acid (agnucastoside A), 6′- O-(6,7-dihydrofoliamenthoyl)mussaenosidic acid (agnucastoside B) and 7- O- trans- p-coumaroyl-6′- O- trans-caffeoyl-8-epiloganic acid (agnucastoside C) in addition to four known iridoids (aucubin, agnuside, mussaenosidic acid and 6′- O- p-hydroxybenzoylmussaenosidic acid) and one known phenylbutanone glucoside (myzodendrone). The structure elucidations were mainly done by spectroscopic methods (1D and 2D NMR spectra) and MS data interpretation. The purified compounds were tested for biological activities against various microorganisms and cancer cell lines. Vitex agnus-castus L. (Verbenaceae) contains three new iridoid glucosides (agnucastosides A–C, 5– 7), besides five known glucosides. All pure compounds ( 1– 8) showed no inhibition against tested microorganisms and cell lines.