Description of Enterococcus canis sp. nov. from dogs and reclassification of Enterococcus porcinus Teixeira et al. 2001 as a junior synonym of Enterococcus villorum Vancanneyt et al. 2001

1 Laboratory of Veterinary Bacteriology and Mycology, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium 2 BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium 3 School of Food Biosciences, University of Reading, Reading RG6 6AP, UK Correspondence E. M. De Graef evelyne.degraef{at}ugent.be Strains from anal swabs and chronic otitis externa in dogs were shown to be phylogenetically related to the Enterococcus faecium species group. They shared a number of phenotypic characteristics with these species, but they could be easily differentiated by biochemical reactions. In addition, the canine strains were unusual in their nearly complete failure to grow on sodium azide-containing enterococci-selective media and in their Voges–Proskauer reactions (usually negative). By using 16S rRNA sequencing and DNA–DNA hybridization of representative strains, as well as tDNA interspacer gene PCR and SDS-PAGE of whole-cell proteins, the group of canine strains was shown to constitute a novel enterococcal species. The name Enterococcus canis sp. nov. is proposed for this species, with LMG 12316 T (=CCUG 46666 T ) as the type strain. Concurrently, the taxonomic situation and nomenclatural position of Enterococcus porcinus were investigated. As no phenotypic or genotypic differences were found between this species and Enterococcus villorum , the name E. porcinus is considered to be a junior synonym of E. villorum . Abbreviations: tDNA-PCR, tRNA intergenic length polymorphism analysis; VP, Voges–Proskauer Published online ahead of print on 13 December 2002 as DOI 10.1099/ijs.0.02549-0. The GenBank/EMBL/DDBJ accession numbers for the 16S DNA sequences reported in this paper are X76177 and AY156090 .