Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs

Summary An Arabidopsis thaliana cDNA bank has been constituted in a Saccharomyces cerevisiae expression vector based on the phosphoglycerate kinase (PGK) promoter and terminator. This bank was used to complement eight S. cerevisiae auxotrophic markers. All of them were corrected. These results confi...

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Veröffentlicht in:	The Plant journal : for cell and molecular biology 1992-05, Vol.2 (3), p.417-422
Hauptverfasser:	Minet, M, Dufour, M.E, Lacroute, F
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acid sequences Arabidopsis - genetics Arabidopsis thaliana Base Sequence cloning Cloning, Molecular dihydroorotic dehydrogenase DNA DNA - genetics enzyme activity gene expression Gene Library gene transfer genetic code genetic complementation Genetic Complementation Test genetic markers Genetic Vectors Molecular Sequence Data mutants Mutation nucleotide sequences oxidoreductases Oxidoreductases - genetics Oxidoreductases Acting on CH-CH Group Donors phosphoglycerate kinase Plants - genetics promoter regions Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics
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Beschreibung
Zusammenfassung:	Summary An Arabidopsis thaliana cDNA bank has been constituted in a Saccharomyces cerevisiae expression vector based on the phosphoglycerate kinase (PGK) promoter and terminator. This bank was used to complement eight S. cerevisiae auxotrophic markers. All of them were corrected. These results confirm the quality of the bank and the feasibility of cloning plant genes by yeast mutant complementation. The cDNA complementing the ura1 yeast mutant was sequenced, analysed and shown to encode a dihydroorotic (DHO) dehydrogenase sequence.
ISSN:	0960-7412 1365-313X
DOI:	10.1046/j.1365-313X.1992.t01-38-00999.x