Simultaneous determination of deuterated and non-deuterated α-tocopherol in human plasma by high-performance liquid chromatography
Labelled tocopherol is used to evaluate its absorption by biodiscriminating the dietary intake from the endogenous tocopherol pool of subject. A normal-phase high-performance liquid chromatographic method is described for the easy separation and quantification of deuterated (d 6) and non-deuterated...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2003-08, Vol.794 (1), p.1-8 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Richelle, Myriam Tavazzi, Isabelle Fay, Laurent Bernard |
| description | Labelled tocopherol is used to evaluate its absorption by biodiscriminating the dietary intake from the endogenous tocopherol pool of subject. A normal-phase high-performance liquid chromatographic method is described for the easy separation and quantification of deuterated (d
6) and non-deuterated α-tocopherol. The α-tocopherol isotopomers were extracted from plasma triacylglycerol-rich lipoproteins in hexane, separated by two EC Nucleosil columns in series with a mobile phase of hexane–isopropanol (659.34:0.786, w/w) running isocratically. The detection of d
6-α-tocopherol was performed by its UV absorbance at 297 nm with a limit of detection of 34 pmol/ml, a limit of quantification of 83 pmol/ml and a range of determination of 34–9905 pmol/ml. Between- and within-assay RSDs were 2.4% (
n=10) and 2.7% (
n=5), respectively. |
| doi_str_mv | 10.1016/S1570-0232(03)00360-X |
| format | Article |
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6-α-tocopherol was performed by its UV absorbance at 297 nm with a limit of detection of 34 pmol/ml, a limit of quantification of 83 pmol/ml and a range of determination of 34–9905 pmol/ml. Between- and within-assay RSDs were 2.4% (
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6) and non-deuterated α-tocopherol. The α-tocopherol isotopomers were extracted from plasma triacylglycerol-rich lipoproteins in hexane, separated by two EC Nucleosil columns in series with a mobile phase of hexane–isopropanol (659.34:0.786, w/w) running isocratically. The detection of d
6-α-tocopherol was performed by its UV absorbance at 297 nm with a limit of detection of 34 pmol/ml, a limit of quantification of 83 pmol/ml and a range of determination of 34–9905 pmol/ml. Between- and within-assay RSDs were 2.4% (
n=10) and 2.7% (
n=5), respectively.</description><subject>alpha-Tocopherol - blood</subject><subject>Aminoacids, peptides. Hormones. Neuropeptides</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Deuterium</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Male</subject><subject>Proteins</subject><subject>Reference Values</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>α-Tocopherol</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM-OFCEQh4nRuOvqI2i4aPSAFs0A3SdjNv5LNvGwmsyNMFC9jelueoE2mbNP5Iv4TLI7Y9abJyo_vqKoj5CnHF5z4OrNJZcaGDSieQniFYBQwLb3yClvtWBCq-39Wv9FTsijnL8DcA1aPCQnvGnblnfNKfl5GaZ1LHbGuGbqsWCawmxLiDONfQ3WmtiCntrZ0znO7J_o9y9WoovLgCmONMx0WCc702W0ebJ0t6dDuBrYgqmPqV44pGO4XoOnbkhxsiVeJbsM-8fkQW_HjE-O5xn59uH91_NP7OLLx8_n7y6Y2zRQmOrlTvGmk1Jb0Sndeokb75UX4HQvW-kdB9SdBM03bdcLVByUVtaD7jRIcUZeHN5dUrxeMRczhexwHA_bGy0k57ptKygPoEsx54S9WVKYbNobDubGvrm1b27UGhDm1r7Z1r5nxwHrbkJ_13XUXYHnR8BmZ8c-VSkh33Gbrmu05JV7e-Cw6vgRMJnsAlaBPiR0xfgY_vOVP_DEpGk</recordid><startdate>20030825</startdate><enddate>20030825</enddate><creator>Richelle, Myriam</creator><creator>Tavazzi, Isabelle</creator><creator>Fay, Laurent Bernard</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030825</creationdate><title>Simultaneous determination of deuterated and non-deuterated α-tocopherol in human plasma by high-performance liquid chromatography</title><author>Richelle, Myriam ; Tavazzi, Isabelle ; Fay, Laurent Bernard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-6f5b6129557a39678d5e4dd6d30c7f585dc10e795071489f3e610676ad0797053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>alpha-Tocopherol - blood</topic><topic>Aminoacids, peptides. Hormones. Neuropeptides</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Deuterium</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Male</topic><topic>Proteins</topic><topic>Reference Values</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>α-Tocopherol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Richelle, Myriam</creatorcontrib><creatorcontrib>Tavazzi, Isabelle</creatorcontrib><creatorcontrib>Fay, Laurent Bernard</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Richelle, Myriam</au><au>Tavazzi, Isabelle</au><au>Fay, Laurent Bernard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous determination of deuterated and non-deuterated α-tocopherol in human plasma by high-performance liquid chromatography</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2003-08-25</date><risdate>2003</risdate><volume>794</volume><issue>1</issue><spage>1</spage><epage>8</epage><pages>1-8</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>Labelled tocopherol is used to evaluate its absorption by biodiscriminating the dietary intake from the endogenous tocopherol pool of subject. A normal-phase high-performance liquid chromatographic method is described for the easy separation and quantification of deuterated (d
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6-α-tocopherol was performed by its UV absorbance at 297 nm with a limit of detection of 34 pmol/ml, a limit of quantification of 83 pmol/ml and a range of determination of 34–9905 pmol/ml. Between- and within-assay RSDs were 2.4% (
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | alpha-Tocopherol - blood Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, High Pressure Liquid - methods Deuterium Fundamental and applied biological sciences. Psychology Humans Male Proteins Reference Values Reproducibility of Results Sensitivity and Specificity Spectrophotometry, Ultraviolet α-Tocopherol |
| title | Simultaneous determination of deuterated and non-deuterated α-tocopherol in human plasma by high-performance liquid chromatography |
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