Simultaneous determination of deuterated and non-deuterated α-tocopherol in human plasma by high-performance liquid chromatography
Labelled tocopherol is used to evaluate its absorption by biodiscriminating the dietary intake from the endogenous tocopherol pool of subject. A normal-phase high-performance liquid chromatographic method is described for the easy separation and quantification of deuterated (d 6) and non-deuterated...
Gespeichert in:
Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2003-08, Vol.794 (1), p.1-8 |
---|---|
Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Labelled tocopherol is used to evaluate its absorption by biodiscriminating the dietary intake from the endogenous tocopherol pool of subject. A normal-phase high-performance liquid chromatographic method is described for the easy separation and quantification of deuterated (d
6) and non-deuterated α-tocopherol. The α-tocopherol isotopomers were extracted from plasma triacylglycerol-rich lipoproteins in hexane, separated by two EC Nucleosil columns in series with a mobile phase of hexane–isopropanol (659.34:0.786, w/w) running isocratically. The detection of d
6-α-tocopherol was performed by its UV absorbance at 297 nm with a limit of detection of 34 pmol/ml, a limit of quantification of 83 pmol/ml and a range of determination of 34–9905 pmol/ml. Between- and within-assay RSDs were 2.4% (
n=10) and 2.7% (
n=5), respectively. |
---|---|
ISSN: | 1570-0232 1873-376X |
DOI: | 10.1016/S1570-0232(03)00360-X |