Age-related differences in the des IGF-I-mediated activation of Akt-1 and p70 S6K in mouse skeletal muscle
The ability of des IGF-I to activate Akt-1 and p70 S6K in skeletal muscle with or without acute endurance exercise was examined in young and old mice. Mice were sacrificed 12 h after a moderate intensity treadmill run following an interperitoneal injection of des-IGF-I or saline. Blood and skeletal...
Gespeichert in:
| Veröffentlicht in: | Mechanisms of ageing and development 2003-07, Vol.124 (7), p.771-778 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 778 |
|---|---|
| container_issue | 7 |
| container_start_page | 771 |
| container_title | Mechanisms of ageing and development |
| container_volume | 124 |
| creator | Li, Minghua Li, Chunmei Parkhouse, Wade S. |
| description | The ability of des IGF-I to activate Akt-1 and p70 S6K in skeletal muscle with or without acute endurance exercise was examined in young and old mice. Mice were sacrificed 12 h after a moderate intensity treadmill run following an interperitoneal injection of des-IGF-I or saline. Blood and skeletal muscle were collected and IGF-I receptor, Akt-1 and p70 S6K protein contents and their phosphorylation status were determined. Injection of des IGF-I similarly decreased plasma glucose concentration in both young (
P |
| doi_str_mv | 10.1016/S0047-6374(03)00124-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73492933</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0047637403001246</els_id><sourcerecordid>73492933</sourcerecordid><originalsourceid>FETCH-LOGICAL-c443t-85fdb498612b621516dfb76f513958b8dc843738085ff242de147fa871ed32773</originalsourceid><addsrcrecordid>eNqFkMluFDEQQC1ERCYDnwDyBUQODt7adp-iUUTCiEg5BM6W2y6Dk14GuycSf49nETnmVFXSq-0h9J7RC0aZ-nJPqdRECS0_U3FOKeOSqFdowYzmRHGmXqPFf-QUnZXyQCsluXqDThk3utGSLdDD6heQDL2bIeCQYoQMo4eC04jn34BDTdc312RNBghpTzk_pyc3p2nEU8Srx5kw7MaAN5rie_V91zlM2wK4PEIPs-vxsC2-h7foJLq-wLtjXKKf119_XH0jt3c366vVLfFSipmYJoZOtkYx3tU_GqZC7LSKDRNtYzoTvJFCC0MrGLnkAZjU0RnNIAiutViiT4e5mzz92UKZ7ZCKh753I9S7rBay5a0QFWwOoM9TKRmi3eQ0uPzXMmp3ku1est0ZtFTYveRaLdGH44JtV608dx2tVuDjEXDFuz5mN_pUnjnZ1g-oqdzlgYOq4ylBtsWnnf6QMvjZhim9cMo_59-WEQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73492933</pqid></control><display><type>article</type><title>Age-related differences in the des IGF-I-mediated activation of Akt-1 and p70 S6K in mouse skeletal muscle</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Li, Minghua ; Li, Chunmei ; Parkhouse, Wade S.</creator><creatorcontrib>Li, Minghua ; Li, Chunmei ; Parkhouse, Wade S.</creatorcontrib><description><![CDATA[The ability of des IGF-I to activate Akt-1 and p70 S6K in skeletal muscle with or without acute endurance exercise was examined in young and old mice. Mice were sacrificed 12 h after a moderate intensity treadmill run following an interperitoneal injection of des-IGF-I or saline. Blood and skeletal muscle were collected and IGF-I receptor, Akt-1 and p70 S6K protein contents and their phosphorylation status were determined. Injection of des IGF-I similarly decreased plasma glucose concentration in both young (
P<0.01) and old mice (
P<0.01) whereas plasma insulin and total IGF-I levels of young and old mice were not significantly changed by des IGF-I. Total IGF-I receptor protein and IGF-1 receptor phosphorylation were lower in aged mice (
P<0.05). Basal phosphorylation of Akt-1 was lower in aged skeletal muscle (
P<0.01) and this was not caused by changes in Akt-1 protein. In both young (
P<0.01) and aged (
P<0.05) mice, des IGF-I significantly increased the phosphorylation of Akt-1 at Ser 473. However, a des IGF-I-mediated increase in the p70 S6K phosphorylation (
P<0.01) was only seen in young mice. Prior exercise decreased the total plasma IGF-I level in the presence of des IGF-I in aged mice. Des IGF-I-mediated Akt-1 and p70 S6K phosphorylation was not changed by exercise in either young or old mice. It is concluded that there was an aging-related resistance at the p70 S6K level in mouse skeletal muscle that could not be restored by prior exercise and this resistance is associated with lower IGF-I receptor number and Akt-1 phosphorylation in the aged skeletal muscle.]]></description><identifier>ISSN: 0047-6374</identifier><identifier>EISSN: 1872-6216</identifier><identifier>DOI: 10.1016/S0047-6374(03)00124-6</identifier><identifier>PMID: 12875741</identifier><identifier>CODEN: MAGDA3</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Aging ; Aging - metabolism ; Akt-1 ; Animals ; Biological and medical sciences ; Blood Glucose - metabolism ; des IGF-I ; Exercise ; Female ; Fundamental and applied biological sciences. Psychology ; Insulin-Like Growth Factor I - metabolism ; Mice ; Mice, Inbred C57BL ; Muscle, Skeletal - metabolism ; p70 S6K ; Phosphorylation ; Protein-Serine-Threonine Kinases - metabolism ; Proto-Oncogene Proteins ; Proto-Oncogene Proteins c-akt ; Receptor, IGF Type 1 - metabolism ; Ribosomal Protein S6 Kinases, 70-kDa - metabolism ; Skeletal muscle ; Striated muscle. Tendons ; Vertebrates: osteoarticular system, musculoskeletal system</subject><ispartof>Mechanisms of ageing and development, 2003-07, Vol.124 (7), p.771-778</ispartof><rights>2003 Elsevier Science Ireland Ltd</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-85fdb498612b621516dfb76f513958b8dc843738085ff242de147fa871ed32773</citedby><cites>FETCH-LOGICAL-c443t-85fdb498612b621516dfb76f513958b8dc843738085ff242de147fa871ed32773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0047637403001246$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14943708$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12875741$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Minghua</creatorcontrib><creatorcontrib>Li, Chunmei</creatorcontrib><creatorcontrib>Parkhouse, Wade S.</creatorcontrib><title>Age-related differences in the des IGF-I-mediated activation of Akt-1 and p70 S6K in mouse skeletal muscle</title><title>Mechanisms of ageing and development</title><addtitle>Mech Ageing Dev</addtitle><description><![CDATA[The ability of des IGF-I to activate Akt-1 and p70 S6K in skeletal muscle with or without acute endurance exercise was examined in young and old mice. Mice were sacrificed 12 h after a moderate intensity treadmill run following an interperitoneal injection of des-IGF-I or saline. Blood and skeletal muscle were collected and IGF-I receptor, Akt-1 and p70 S6K protein contents and their phosphorylation status were determined. Injection of des IGF-I similarly decreased plasma glucose concentration in both young (
P<0.01) and old mice (
P<0.01) whereas plasma insulin and total IGF-I levels of young and old mice were not significantly changed by des IGF-I. Total IGF-I receptor protein and IGF-1 receptor phosphorylation were lower in aged mice (
P<0.05). Basal phosphorylation of Akt-1 was lower in aged skeletal muscle (
P<0.01) and this was not caused by changes in Akt-1 protein. In both young (
P<0.01) and aged (
P<0.05) mice, des IGF-I significantly increased the phosphorylation of Akt-1 at Ser 473. However, a des IGF-I-mediated increase in the p70 S6K phosphorylation (
P<0.01) was only seen in young mice. Prior exercise decreased the total plasma IGF-I level in the presence of des IGF-I in aged mice. Des IGF-I-mediated Akt-1 and p70 S6K phosphorylation was not changed by exercise in either young or old mice. It is concluded that there was an aging-related resistance at the p70 S6K level in mouse skeletal muscle that could not be restored by prior exercise and this resistance is associated with lower IGF-I receptor number and Akt-1 phosphorylation in the aged skeletal muscle.]]></description><subject>Aging</subject><subject>Aging - metabolism</subject><subject>Akt-1</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Glucose - metabolism</subject><subject>des IGF-I</subject><subject>Exercise</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Insulin-Like Growth Factor I - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Muscle, Skeletal - metabolism</subject><subject>p70 S6K</subject><subject>Phosphorylation</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>Proto-Oncogene Proteins</subject><subject>Proto-Oncogene Proteins c-akt</subject><subject>Receptor, IGF Type 1 - metabolism</subject><subject>Ribosomal Protein S6 Kinases, 70-kDa - metabolism</subject><subject>Skeletal muscle</subject><subject>Striated muscle. Tendons</subject><subject>Vertebrates: osteoarticular system, musculoskeletal system</subject><issn>0047-6374</issn><issn>1872-6216</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMluFDEQQC1ERCYDnwDyBUQODt7adp-iUUTCiEg5BM6W2y6Dk14GuycSf49nETnmVFXSq-0h9J7RC0aZ-nJPqdRECS0_U3FOKeOSqFdowYzmRHGmXqPFf-QUnZXyQCsluXqDThk3utGSLdDD6heQDL2bIeCQYoQMo4eC04jn34BDTdc312RNBghpTzk_pyc3p2nEU8Srx5kw7MaAN5rie_V91zlM2wK4PEIPs-vxsC2-h7foJLq-wLtjXKKf119_XH0jt3c366vVLfFSipmYJoZOtkYx3tU_GqZC7LSKDRNtYzoTvJFCC0MrGLnkAZjU0RnNIAiutViiT4e5mzz92UKZ7ZCKh753I9S7rBay5a0QFWwOoM9TKRmi3eQ0uPzXMmp3ku1est0ZtFTYveRaLdGH44JtV608dx2tVuDjEXDFuz5mN_pUnjnZ1g-oqdzlgYOq4ylBtsWnnf6QMvjZhim9cMo_59-WEQ</recordid><startdate>20030701</startdate><enddate>20030701</enddate><creator>Li, Minghua</creator><creator>Li, Chunmei</creator><creator>Parkhouse, Wade S.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030701</creationdate><title>Age-related differences in the des IGF-I-mediated activation of Akt-1 and p70 S6K in mouse skeletal muscle</title><author>Li, Minghua ; Li, Chunmei ; Parkhouse, Wade S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-85fdb498612b621516dfb76f513958b8dc843738085ff242de147fa871ed32773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Aging</topic><topic>Aging - metabolism</topic><topic>Akt-1</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Glucose - metabolism</topic><topic>des IGF-I</topic><topic>Exercise</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Insulin-Like Growth Factor I - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Muscle, Skeletal - metabolism</topic><topic>p70 S6K</topic><topic>Phosphorylation</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>Proto-Oncogene Proteins</topic><topic>Proto-Oncogene Proteins c-akt</topic><topic>Receptor, IGF Type 1 - metabolism</topic><topic>Ribosomal Protein S6 Kinases, 70-kDa - metabolism</topic><topic>Skeletal muscle</topic><topic>Striated muscle. Tendons</topic><topic>Vertebrates: osteoarticular system, musculoskeletal system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Minghua</creatorcontrib><creatorcontrib>Li, Chunmei</creatorcontrib><creatorcontrib>Parkhouse, Wade S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Mechanisms of ageing and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Minghua</au><au>Li, Chunmei</au><au>Parkhouse, Wade S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Age-related differences in the des IGF-I-mediated activation of Akt-1 and p70 S6K in mouse skeletal muscle</atitle><jtitle>Mechanisms of ageing and development</jtitle><addtitle>Mech Ageing Dev</addtitle><date>2003-07-01</date><risdate>2003</risdate><volume>124</volume><issue>7</issue><spage>771</spage><epage>778</epage><pages>771-778</pages><issn>0047-6374</issn><eissn>1872-6216</eissn><coden>MAGDA3</coden><abstract><![CDATA[The ability of des IGF-I to activate Akt-1 and p70 S6K in skeletal muscle with or without acute endurance exercise was examined in young and old mice. Mice were sacrificed 12 h after a moderate intensity treadmill run following an interperitoneal injection of des-IGF-I or saline. Blood and skeletal muscle were collected and IGF-I receptor, Akt-1 and p70 S6K protein contents and their phosphorylation status were determined. Injection of des IGF-I similarly decreased plasma glucose concentration in both young (
P<0.01) and old mice (
P<0.01) whereas plasma insulin and total IGF-I levels of young and old mice were not significantly changed by des IGF-I. Total IGF-I receptor protein and IGF-1 receptor phosphorylation were lower in aged mice (
P<0.05). Basal phosphorylation of Akt-1 was lower in aged skeletal muscle (
P<0.01) and this was not caused by changes in Akt-1 protein. In both young (
P<0.01) and aged (
P<0.05) mice, des IGF-I significantly increased the phosphorylation of Akt-1 at Ser 473. However, a des IGF-I-mediated increase in the p70 S6K phosphorylation (
P<0.01) was only seen in young mice. Prior exercise decreased the total plasma IGF-I level in the presence of des IGF-I in aged mice. Des IGF-I-mediated Akt-1 and p70 S6K phosphorylation was not changed by exercise in either young or old mice. It is concluded that there was an aging-related resistance at the p70 S6K level in mouse skeletal muscle that could not be restored by prior exercise and this resistance is associated with lower IGF-I receptor number and Akt-1 phosphorylation in the aged skeletal muscle.]]></abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>12875741</pmid><doi>10.1016/S0047-6374(03)00124-6</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0047-6374 |
| ispartof | Mechanisms of ageing and development, 2003-07, Vol.124 (7), p.771-778 |
| issn | 0047-6374 1872-6216 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73492933 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aging Aging - metabolism Akt-1 Animals Biological and medical sciences Blood Glucose - metabolism des IGF-I Exercise Female Fundamental and applied biological sciences. Psychology Insulin-Like Growth Factor I - metabolism Mice Mice, Inbred C57BL Muscle, Skeletal - metabolism p70 S6K Phosphorylation Protein-Serine-Threonine Kinases - metabolism Proto-Oncogene Proteins Proto-Oncogene Proteins c-akt Receptor, IGF Type 1 - metabolism Ribosomal Protein S6 Kinases, 70-kDa - metabolism Skeletal muscle Striated muscle. Tendons Vertebrates: osteoarticular system, musculoskeletal system |
| title | Age-related differences in the des IGF-I-mediated activation of Akt-1 and p70 S6K in mouse skeletal muscle |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T06%3A22%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Age-related%20differences%20in%20the%20des%20IGF-I-mediated%20activation%20of%20Akt-1%20and%20p70%20S6K%20in%20mouse%20skeletal%20muscle&rft.jtitle=Mechanisms%20of%20ageing%20and%20development&rft.au=Li,%20Minghua&rft.date=2003-07-01&rft.volume=124&rft.issue=7&rft.spage=771&rft.epage=778&rft.pages=771-778&rft.issn=0047-6374&rft.eissn=1872-6216&rft.coden=MAGDA3&rft_id=info:doi/10.1016/S0047-6374(03)00124-6&rft_dat=%3Cproquest_cross%3E73492933%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73492933&rft_id=info:pmid/12875741&rft_els_id=S0047637403001246&rfr_iscdi=true |