SP600125, an inhibitor of c-jun N-terminal kinase, activates CREB by a p38 MAPK-mediated pathway

SP600125, an inhibitor of c-jun N-terminal kinase, activates CREB by a p38 MAPK-mediated pathway

SP600125, an anthrapyrazolone inhibitor of c-jun N-terminal kinase (JNK), has been used to characterize the role of JNK in apoptotic pathways. In this study, we have demonstrated an additional novel anti-apoptotic action of this inhibitor in MIN6 cells, a mouse beta cell line. SP600125 induced CREB-...

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Veröffentlicht in:Biochemical and biophysical research communications 2003-08, Vol.307 (4), p.855-860
Hauptverfasser: Vaishnav, Dhara, Jambal, Purevsuren, Reusch, Jane E.-B, Pugazhenthi, Subbiah
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Anthracenes - pharmacology
Cyclic AMP Response Element-Binding Protein - chemistry
Cyclic AMP Response Element-Binding Protein - metabolism
Enzyme Inhibitors - pharmacology
Imidazoles - pharmacology
JNK Mitogen-Activated Protein Kinases
MAP Kinase Signaling System
Mice
Mitogen-Activated Protein Kinases - antagonists & inhibitors
Mitogen-Activated Protein Kinases - metabolism
p38 Mitogen-Activated Protein Kinases
Phosphorylation
Promoter Regions, Genetic
Pyridines - pharmacology
Serine - metabolism
Transcriptional Activation
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Zusammenfassung:SP600125, an anthrapyrazolone inhibitor of c-jun N-terminal kinase (JNK), has been used to characterize the role of JNK in apoptotic pathways. In this study, we have demonstrated an additional novel anti-apoptotic action of this inhibitor in MIN6 cells, a mouse beta cell line. SP600125 induced CREB-dependent promoter activation by 2.8-fold at 20 μM, the concentration at which it inhibited c-jun-dependent promoter activation by 51%. There was a significant ( P
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(03)01287-7