Interaction of cyanine dyes with nucleic acids: XXXI. Using of polymethine cyanine dyes for the visualization of DNA in agarose gels

Interaction of cyanine dyes with nucleic acids: XXXI. Using of polymethine cyanine dyes for the visualization of DNA in agarose gels

Fifteen polymethine cyanine dyes were studied as fluorescent stains for DNA in electrophoretic gels. Among studied cyanines, two dyes CPent V and CCyan 2-O most effectively visualized covalently closed and linear double-stranded DNA molecules in gels under standard conditions using UV-illumination,...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of biochemical and biophysical methods 2003-07, Vol.57 (1), p.35-43
Hauptverfasser: Matselyukh, B.P., Yarmoluk, S.M., Matselyukh, A.B., Kovalska, V.B., Kocheshev, I.O., Kryvorotenko, D.V., Lukashov, S.S.
Format: Artikel
Sprache:eng
Schlagworte:
Carbocyanines - chemistry
Cyanine dyes
DNA
DNA - analysis
DNA - chemistry
Electrophoresis, Agar Gel - methods
Ethidium - chemistry
Fluorescent Dyes - chemistry
Fluorescent probes
Molecular Structure
Nucleic Acids - chemistry
Staining and Labeling
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Fifteen polymethine cyanine dyes were studied as fluorescent stains for DNA in electrophoretic gels. Among studied cyanines, two dyes CPent V and CCyan 2-O most effectively visualized covalently closed and linear double-stranded DNA molecules in gels under standard conditions using UV-illumination, green filter and black-and-white photo film. Ethidium bromide was 1.2–1.6 times more effective as compared to cyanine dyes in staining of DNA in the concentration range of 8–18 ng, while studied cyanines were more sensitive to DNA quantity above 50 ng.
ISSN:0165-022X
1872-857X
DOI:10.1016/S0165-022X(03)00036-8