Semi-Automated Synthesis and Screening of a Large Library of Degradable Cationic Polymers for Gene Delivery

Higher delivery efficiency than conventional nonviral systems (such as poly(ethyleneimine)) have been identified in 46 polymers through cell‐based screening of a large, 2350‐member library by a high‐throughput, semi‐automated process. The transfection potential of the polymers was assessed by testing their ability to deliver luciferase expressing plasmid and green fluorescent protein plasmid (see picture) to a monkey kidney fibroblast cell line.