Hydrogen peroxide biosensor based on microperoxidase-11 entrapped in lipid membrane

A highly catalytic activity microperoxidase-11 (MP-11) biosensor for H 2O 2 was developed to immobilizing the heme peptide in didodecyldimethylammonium bromide (DDAB) lipid membrane. The enzyme electrode thus obtained responded to H 2O 2 without electron mediator or promoter, at a potential of +0.10 V versus Ag ∣ AgCl. A linear calibration curve is obtained over the range from 2.0×10 −5 to 2.4×10 −3 M. The biosensor responds to hydrogen peroxide in 15 s and has a detection limit of 8×10 −7 M (S/N=3) Providing a natural environment with lipid membrane for protein immobilization and maintenance of protein functions is a suitable option for the design of biosensors.