Immobilization of mold and bacterial amylases on silica carriers

The immobilization of α‐amylase and glucoamylase was investigated by several coupling methods on silica carriers, different types of Silokhroms, and silica gels. The most active immobilized mold and bacterial α‐amylases and mold glucoamylase were obtained with titanium salts. These activities were twice the value of that obtained by glutaraldehyde or azo coupling. The half‐lives of A. oryzae α‐amylase, B. subtilis α‐amylase, and A. niger glucoamylase, immobilized to silica carriers at 45°C and under continuous operation at a high concentration of substrate, were 14, 35, and 65 days, respectively.