Large Area Protein Patterning Reveals Nanoscale Control of Focal Adhesion Development

Focal adhesion development in cells adherent to surface bound fibronectin presented as 200, 500, or 1000 nm diameter circular patches or as homogeneous controls is studied by fluorescence and scanning electron microscopy. Fundamental cellular processes such as adhesion, spreading, focal adhesion and...

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Veröffentlicht in:	Nano letters 2010-02, Vol.10 (2), p.686-694
Hauptverfasser:	Malmström, Jenny, Christensen, Brian, Jakobsen, Hans P, Lovmand, Jette, Foldbjerg, Rasmus, Sørensen, Esben S, Sutherland, Duncan S
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biocompatible Materials - chemistry Cell Adhesion Colloids - chemistry Condensed matter: structure, mechanical and thermal properties Cross-disciplinary physics: materials science rheology Exact sciences and technology Fibronectins - chemistry Focal Adhesions Humans Ligands Materials science Mechanical and acoustical properties of condensed matter Mechanical properties of nanoscale materials Methods of nanofabrication Microscopy, Electron, Scanning - methods Microscopy, Fluorescence - methods Nanoparticles - chemistry Nanoscale pattern formation Nanostructures Nanotechnology - methods Physics Proteins - chemistry Rhodamines - chemistry
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Beschreibung
Zusammenfassung:	Focal adhesion development in cells adherent to surface bound fibronectin presented as 200, 500, or 1000 nm diameter circular patches or as homogeneous controls is studied by fluorescence and scanning electron microscopy. Fundamental cellular processes such as adhesion, spreading, focal adhesion and stress fiber formation are shown to be dependent on the spatial distribution of ligands at this scale. Large area samples enable the study of whole cell populations and opens for new potential applications.
ISSN:	1530-6984 1530-6992
DOI:	10.1021/nl903875r