Viral Inhibition Assay: A CD8 T Cell Neutralization Assay for Use in Clinical Trials of HIV-1 Vaccine Candidates

We have characterized an assay measuring CD8 T cell-mediated inhibition of human immunodeficiency virus (HIV) type 1 replication, demonstrating specificity and reproducibility and employing a panel of primary HIV-1 isolates. The assay uses relatively simple autologous cell culture and enzyme-linked...

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Veröffentlicht in:	The Journal of infectious diseases 2010-03, Vol.201 (5), p.720-729
Hauptverfasser:	Spentzou, Aggeliki, Bergin, Philip, Gill, Dilbinder, Cheeseman, Hannah, Ashraf, Ambreen, Kaltsidis, Harry, Cashin-Cox, Michelle, Anjarwalla, Insiyah, Steel, Alan, Higgs, Christopher, Pozniak, Anton, Piechocka-Trocha, Alicja, Wong, Johnson, Anzala, Omu, Karita, Etienne, Dally, Len, Gotch, Frances, Walker, Bruce, Gilmour, Jill, Hayes, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenovirus Adenoviruses, Human - genetics Adult Aged AIDS Vaccines - genetics AIDS Vaccines - immunology Applied microbiology Biological and medical sciences CD8-Positive T-Lymphocytes - immunology Cells, Cultured Clinical trials Cultured cells Enzyme linked immunospot assay Enzyme-Linked Immunosorbent Assay - methods Female Fundamental and applied biological sciences. Psychology Genetic Vectors HIV HIV 1 HIV-1 - immunology HIV/AIDS Human immunodeficiency virus 1 Humans Infections Infectious diseases Lymphocytes Male Medical sciences Microbiology Middle Aged Miscellaneous Neutralization Tests - methods Placebos Reproducibility of Results Sensitivity and Specificity Vaccination Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Virology Virus Replication - immunology Viruses
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Beschreibung
Zusammenfassung:	We have characterized an assay measuring CD8 T cell-mediated inhibition of human immunodeficiency virus (HIV) type 1 replication, demonstrating specificity and reproducibility and employing a panel of primary HIV-1 isolates. The assay uses relatively simple autologous cell culture and enzyme-linked immunosorbent assay, avoids generation of T cell clones, and can be performed with
ISSN:	0022-1899 1573-6613 1537-6613
DOI:	10.1086/650492