Optical collection efficiency function in single-molecule detection experiments

The optical collection efficiency function for an optical system such as that used in single-molecule detection experiments is studied. Closed analytical expressions based on a geometrical optics approximation are presented. Comparison is made with exact wave optics calculations.

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Bibliographische Detailangaben
Veröffentlicht in:	Applied Optics 1997-08, Vol.36 (22), p.5298-5302
Hauptverfasser:	Enderlein, J, Ambrose, W P
Format:	Artikel
Sprache:	eng
Schlagworte:	DIFFRACTION EFFICIENCY FLUORESCENCE SPECTROSCOPY FRESNEL COEFFICIENT INSTRUMENTATION, INCLUDING NUCLEAR AND PARTICLE DETECTORS LASER SPECTROSCOPY MOLECULES OPTICS PHYSICS SENSITIVITY ANALYSIS
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Beschreibung
Zusammenfassung:	The optical collection efficiency function for an optical system such as that used in single-molecule detection experiments is studied. Closed analytical expressions based on a geometrical optics approximation are presented. Comparison is made with exact wave optics calculations.
ISSN:	1559-128X 0003-6935 1539-4522
DOI:	10.1364/AO.36.005298