Effect of Troglitazone on Expression of Adhesion Molecules and eNOS in Human Saphenous Vein Graft

To investigate whether peroxisome proliferators-activated receptor-γ (PPARγ) ligand Troglitazone can reduce endothelial injury and activation during storage of harvested saphenous vein grafts. Segments of human saphenous vein graft were collected from 9 patients undergoing coronary bypass surgery an...

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Veröffentlicht in:Journal of Huazhong University of Science and Technology. Medical sciences 2007-12, Vol.27 (6), p.657-659
1. Verfasser: 陈澍 胡志伟 张凯伦 苏伟 孙宗全
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Sprache:eng
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Zusammenfassung:To investigate whether peroxisome proliferators-activated receptor-γ (PPARγ) ligand Troglitazone can reduce endothelial injury and activation during storage of harvested saphenous vein grafts. Segments of human saphenous vein graft were collected from 9 patients undergoing coronary bypass surgery and then divided into two equal parts of control and test specimens, were stored in ei- ther heparinized blood (control group) or heparinized blood containing 20 μmol/L troglitazone (test group) for 1 h at room temperature. Tissue distribution and protein expression of VCAM-I, ICAM-I, and endothelial nitric oxide synthase (eNOS) were compared using immunohistochemistry and Western blot analysis. Myeloperoxidase (MPO) activity, a marker of neutrophil sequestration in human saphenous vein grafts, was also measured in each group. The expression of ICAM-1 (753±132 versus 7201±934; P〈0.01) , VCAM-1 (3731±294 versus 8292±793; P〈0.01), and MPO activity (1.52±0.42 U/g, 5.04±1.26 U/g P〈0.01) were significantly lower in test group. In contract, eNOS expression (7983±834 versus 3989±1008; P〈0.01) was significantly higher in test group. PPARγ ligand troglitazone might reduce endothelial injury during the storage period of human saphenous vein grafts.
ISSN:1672-0733
1993-1352
DOI:10.1007/s11596-007-0609-9