Real-time observation of single-molecule fluorescence in microdroplet streams

Real-time observation of single-molecule fluorescence in microdroplet streams

We report real-time observation of fluorescence bursts from individual Rhodamine 6G molecules in streams of microdroplets (peak signal-to-noise ratios, approximately 30) whose trajectories are constrained with a linear electric quadrupole. This approach offers a reasonable dynamic range in droplet s...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Optics Letters 1997-08, Vol.22 (16), p.1265-1267
Hauptverfasser: Barnes, M D, Lermer, N, Kung, C Y, Whitten, W B, Ramsey, J M, Hill, S C
Format: Artikel
Sprache:eng
Schlagworte:
CAVITY RESONATORS
DROPLETS
ENGINEERING NOT INCLUDED IN OTHER CATEGORIES
FLUORESCENCE
MOLECULES
RHODAMINES
SIGNAL-TO-NOISE RATIO
STIMULATED EMISSION
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1267
container_issue 16
container_start_page 1265
container_title Optics Letters
container_volume 22
creator Barnes, M D
Lermer, N
Kung, C Y
Whitten, W B
Ramsey, J M
Hill, S C
description We report real-time observation of fluorescence bursts from individual Rhodamine 6G molecules in streams of microdroplets (peak signal-to-noise ratios, approximately 30) whose trajectories are constrained with a linear electric quadrupole. This approach offers a reasonable dynamic range in droplet size (3- 12-microm diameter) with
doi_str_mv 10.1364/ol.22.001265
format Article
fullrecord <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_proquest_miscellaneous_734227871</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>734227871</sourcerecordid><originalsourceid>FETCH-LOGICAL-c380t-7a16821f0734479a48bf1015136f0d731de524af56164e8b0606dcc2fe8a03bf3</originalsourceid><addsrcrecordid>eNpFkEtLxDAUhYMozvjYuZa6cmPHvJtZyuALRgTRdUjTG42kzZikgv_eygy4upuPc8_5EDojeEGY5NcxLChdYEyoFHtoTgRb1rxZ8n00x4TLeimWdIaOcv7EGMuGsUM0I4oooYiYo6cXMKEuvocqthnStyk-DlV0VfbDe4C6jwHsGKByYYwJsoXBQuWHqvc2xS7FTYBS5ZLA9PkEHTgTMpzu7jF6u7t9XT3U6-f7x9XNurZM4VI3hkhFicMN41NVw1XrCCZi2uNw1zDSgaDcOCGJ5KBaLLHsrKUOlMGsdewYXWxzYy5eZ-sL2A8bhwFs0YIrRdnEXG6ZTYpfI-Siez-1D8EMEMesp9-UNqohE3m1JadBOSdwepN8b9KPJlj_OdbPa02p3jqe8PNd8Nj20P3DO6nsF3tpdpY</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>734227871</pqid></control><display><type>article</type><title>Real-time observation of single-molecule fluorescence in microdroplet streams</title><source>Optica Publishing Group Journals</source><creator>Barnes, M D ; Lermer, N ; Kung, C Y ; Whitten, W B ; Ramsey, J M ; Hill, S C</creator><creatorcontrib>Barnes, M D ; Lermer, N ; Kung, C Y ; Whitten, W B ; Ramsey, J M ; Hill, S C ; Oak Ridge National Laboratory</creatorcontrib><description>We report real-time observation of fluorescence bursts from individual Rhodamine 6G molecules in streams of microdroplets (peak signal-to-noise ratios, approximately 30) whose trajectories are constrained with a linear electric quadrupole. This approach offers a reasonable dynamic range in droplet size (3- 12-microm diameter) with &lt;1% shot-to-shot size fluctuations and sensitivity comparable with that of droplet levitation techniques with at least 10(3) higher analysis rates. Applications to the study of single-molecule microcavity effects and stimulated emission are discussed.</description><identifier>ISSN: 0146-9592</identifier><identifier>EISSN: 1539-4794</identifier><identifier>DOI: 10.1364/ol.22.001265</identifier><identifier>PMID: 18185815</identifier><language>eng</language><publisher>United States</publisher><subject>CAVITY RESONATORS ; DROPLETS ; ENGINEERING NOT INCLUDED IN OTHER CATEGORIES ; FLUORESCENCE ; MOLECULES ; RHODAMINES ; SIGNAL-TO-NOISE RATIO ; STIMULATED EMISSION</subject><ispartof>Optics Letters, 1997-08, Vol.22 (16), p.1265-1267</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-7a16821f0734479a48bf1015136f0d731de524af56164e8b0606dcc2fe8a03bf3</citedby><cites>FETCH-LOGICAL-c380t-7a16821f0734479a48bf1015136f0d731de524af56164e8b0606dcc2fe8a03bf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,885,3258,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18185815$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/548823$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Barnes, M D</creatorcontrib><creatorcontrib>Lermer, N</creatorcontrib><creatorcontrib>Kung, C Y</creatorcontrib><creatorcontrib>Whitten, W B</creatorcontrib><creatorcontrib>Ramsey, J M</creatorcontrib><creatorcontrib>Hill, S C</creatorcontrib><creatorcontrib>Oak Ridge National Laboratory</creatorcontrib><title>Real-time observation of single-molecule fluorescence in microdroplet streams</title><title>Optics Letters</title><addtitle>Opt Lett</addtitle><description>We report real-time observation of fluorescence bursts from individual Rhodamine 6G molecules in streams of microdroplets (peak signal-to-noise ratios, approximately 30) whose trajectories are constrained with a linear electric quadrupole. This approach offers a reasonable dynamic range in droplet size (3- 12-microm diameter) with &lt;1% shot-to-shot size fluctuations and sensitivity comparable with that of droplet levitation techniques with at least 10(3) higher analysis rates. Applications to the study of single-molecule microcavity effects and stimulated emission are discussed.</description><subject>CAVITY RESONATORS</subject><subject>DROPLETS</subject><subject>ENGINEERING NOT INCLUDED IN OTHER CATEGORIES</subject><subject>FLUORESCENCE</subject><subject>MOLECULES</subject><subject>RHODAMINES</subject><subject>SIGNAL-TO-NOISE RATIO</subject><subject>STIMULATED EMISSION</subject><issn>0146-9592</issn><issn>1539-4794</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNpFkEtLxDAUhYMozvjYuZa6cmPHvJtZyuALRgTRdUjTG42kzZikgv_eygy4upuPc8_5EDojeEGY5NcxLChdYEyoFHtoTgRb1rxZ8n00x4TLeimWdIaOcv7EGMuGsUM0I4oooYiYo6cXMKEuvocqthnStyk-DlV0VfbDe4C6jwHsGKByYYwJsoXBQuWHqvc2xS7FTYBS5ZLA9PkEHTgTMpzu7jF6u7t9XT3U6-f7x9XNurZM4VI3hkhFicMN41NVw1XrCCZi2uNw1zDSgaDcOCGJ5KBaLLHsrKUOlMGsdewYXWxzYy5eZ-sL2A8bhwFs0YIrRdnEXG6ZTYpfI-Siez-1D8EMEMesp9-UNqohE3m1JadBOSdwepN8b9KPJlj_OdbPa02p3jqe8PNd8Nj20P3DO6nsF3tpdpY</recordid><startdate>19970815</startdate><enddate>19970815</enddate><creator>Barnes, M D</creator><creator>Lermer, N</creator><creator>Kung, C Y</creator><creator>Whitten, W B</creator><creator>Ramsey, J M</creator><creator>Hill, S C</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19970815</creationdate><title>Real-time observation of single-molecule fluorescence in microdroplet streams</title><author>Barnes, M D ; Lermer, N ; Kung, C Y ; Whitten, W B ; Ramsey, J M ; Hill, S C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-7a16821f0734479a48bf1015136f0d731de524af56164e8b0606dcc2fe8a03bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>CAVITY RESONATORS</topic><topic>DROPLETS</topic><topic>ENGINEERING NOT INCLUDED IN OTHER CATEGORIES</topic><topic>FLUORESCENCE</topic><topic>MOLECULES</topic><topic>RHODAMINES</topic><topic>SIGNAL-TO-NOISE RATIO</topic><topic>STIMULATED EMISSION</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barnes, M D</creatorcontrib><creatorcontrib>Lermer, N</creatorcontrib><creatorcontrib>Kung, C Y</creatorcontrib><creatorcontrib>Whitten, W B</creatorcontrib><creatorcontrib>Ramsey, J M</creatorcontrib><creatorcontrib>Hill, S C</creatorcontrib><creatorcontrib>Oak Ridge National Laboratory</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Optics Letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barnes, M D</au><au>Lermer, N</au><au>Kung, C Y</au><au>Whitten, W B</au><au>Ramsey, J M</au><au>Hill, S C</au><aucorp>Oak Ridge National Laboratory</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Real-time observation of single-molecule fluorescence in microdroplet streams</atitle><jtitle>Optics Letters</jtitle><addtitle>Opt Lett</addtitle><date>1997-08-15</date><risdate>1997</risdate><volume>22</volume><issue>16</issue><spage>1265</spage><epage>1267</epage><pages>1265-1267</pages><issn>0146-9592</issn><eissn>1539-4794</eissn><abstract>We report real-time observation of fluorescence bursts from individual Rhodamine 6G molecules in streams of microdroplets (peak signal-to-noise ratios, approximately 30) whose trajectories are constrained with a linear electric quadrupole. This approach offers a reasonable dynamic range in droplet size (3- 12-microm diameter) with &lt;1% shot-to-shot size fluctuations and sensitivity comparable with that of droplet levitation techniques with at least 10(3) higher analysis rates. Applications to the study of single-molecule microcavity effects and stimulated emission are discussed.</abstract><cop>United States</cop><pmid>18185815</pmid><doi>10.1364/ol.22.001265</doi><tpages>3</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0146-9592
ispartof Optics Letters, 1997-08, Vol.22 (16), p.1265-1267
issn 0146-9592
1539-4794
language eng
recordid cdi_proquest_miscellaneous_734227871
source Optica Publishing Group Journals
subjects CAVITY RESONATORS
DROPLETS
ENGINEERING NOT INCLUDED IN OTHER CATEGORIES
FLUORESCENCE
MOLECULES
RHODAMINES
SIGNAL-TO-NOISE RATIO
STIMULATED EMISSION
title Real-time observation of single-molecule fluorescence in microdroplet streams
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T18%3A21%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Real-time%20observation%20of%20single-molecule%20fluorescence%20in%20microdroplet%20streams&rft.jtitle=Optics%20Letters&rft.au=Barnes,%20M%20D&rft.aucorp=Oak%20Ridge%20National%20Laboratory&rft.date=1997-08-15&rft.volume=22&rft.issue=16&rft.spage=1265&rft.epage=1267&rft.pages=1265-1267&rft.issn=0146-9592&rft.eissn=1539-4794&rft_id=info:doi/10.1364/ol.22.001265&rft_dat=%3Cproquest_osti_%3E734227871%3C/proquest_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=734227871&rft_id=info:pmid/18185815&rfr_iscdi=true