Real-time observation of single-molecule fluorescence in microdroplet streams

Real-time observation of single-molecule fluorescence in microdroplet streams

We report real-time observation of fluorescence bursts from individual Rhodamine 6G molecules in streams of microdroplets (peak signal-to-noise ratios, approximately 30) whose trajectories are constrained with a linear electric quadrupole. This approach offers a reasonable dynamic range in droplet s...

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Veröffentlicht in:Optics Letters 1997-08, Vol.22 (16), p.1265-1267
Hauptverfasser: Barnes, M D, Lermer, N, Kung, C Y, Whitten, W B, Ramsey, J M, Hill, S C
Format: Artikel
Sprache:eng
Schlagworte:
CAVITY RESONATORS
DROPLETS
ENGINEERING NOT INCLUDED IN OTHER CATEGORIES
FLUORESCENCE
MOLECULES
RHODAMINES
SIGNAL-TO-NOISE RATIO
STIMULATED EMISSION
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Zusammenfassung:We report real-time observation of fluorescence bursts from individual Rhodamine 6G molecules in streams of microdroplets (peak signal-to-noise ratios, approximately 30) whose trajectories are constrained with a linear electric quadrupole. This approach offers a reasonable dynamic range in droplet size (3- 12-microm diameter) with
ISSN:0146-9592
1539-4794
DOI:10.1364/ol.22.001265