Chemotaxis of human blood monocytes toward endothelin-1 and the influence of calcium channel blockers

Chemotaxis of human blood monocytes toward endothelin-1 and the influence of calcium channel blockers

The adherence of monocytes to the arterial endothelium followed by its migration into the arterial intima is the earliest event in atherogenesis. The vasoconstrictive peptide, Endothelin-1 (ET-1), is elevated in patients with atherosclerosis. We were interested to know whether ET-1 was a chemoattrac...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biochemical and biophysical research communications 1992-12, Vol.189 (2), p.994-1000
Hauptverfasser: Hanggono Achmad, Tri, Rao, Govind S.
Format: Artikel
Sprache:eng
Schlagworte:
Aspirin - pharmacology
Biological and medical sciences
Calcium Channel Blockers - pharmacology
Cell physiology
Chemotaxis, Leukocyte - drug effects
Chemotaxis, Leukocyte - physiology
Diltiazem - pharmacology
Dose-Response Relationship, Drug
Endothelins - pharmacology
Fundamental and applied biological sciences. Psychology
Humans
In Vitro Techniques
Indomethacin - pharmacology
Kinetics
Molecular and cellular biology
Monocytes - drug effects
Monocytes - physiology
Motility and taxis
Neomycin - pharmacology
Nifedipine - pharmacology
Probucol - pharmacology
Thioctic Acid - pharmacology
Verapamil - pharmacology
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The adherence of monocytes to the arterial endothelium followed by its migration into the arterial intima is the earliest event in atherogenesis. The vasoconstrictive peptide, Endothelin-1 (ET-1), is elevated in patients with atherosclerosis. We were interested to know whether ET-1 was a chemoattractant for blood monocytes. Using the modified membrane filter technique for chemotaxsis assessment, ET-1 increased monocyte chemotaxis in a dose-dependent manner. Ca 2+ channel blockers, Nifedipine, Diltiazem and Verapamil (5 μM), reduced ET-1 chemotaxsis more than 60% ( P < 0.001). Aspirin and Indomethacin (1 mM and 100 μM, respectively) reduced migration by 23% ( P
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(92)92302-E