Novel 3′-O-Fluorescently Modified Nucleotides for Reversible Termination of DNA Synthesis

Serving a double purpose: 3′‐O‐fluorophore‐labeled dTTPs were synthesized and used as reversible terminators of DNA polymerization. Successful incorporation of nucleotides with a bulky 3′‐O‐fluorophore and detection of fluorescence for base calling can potentially be used to improve sequencing‐by‐synthesis technology.