An unsaturated phosphonic acid analogue of phosphatidylethanolamine and its activity in blood‐clotting systems

An unsaturated phosphonolipid analogous to phosphatidylethanolamine,rac‐dioleoylglyceryl(2‐aminoethyl)phosphonate, was synthesized by a general method introduced by Baer for similar saturated substances. An improvement was made in the preparation of the phthalimidoethyl‐phosphonic acid precursor. The phosphonolipid was purified by DEAE cellulose and silicic acid chromatography. It was tested by comparison with synthetic phosphatidyl (dioleoyl) ethanolamine and phosphatidyl(dilinoleoyl) ethanolamine in the Hicks‐Pitney test and in a test for prothrombin conversion by using purified blood coagulation factors. In both tests it had more acceleratory activity than the synthetic phosphatidylethanolamines.