Separation of cholesterol‐desmosterol acetates by thin‐layer and column chromatography on silica gel G‐silver nitrate
A chromatographic method, using columns packed with Celite‐Silica Gel G impregnated with silver nitrate, has been developed for the separation of cholesterol from desmosterol (24‐dehydrocholesterol). The system employed allows a complete separation of the two sterols as acetates, as determined by ga...
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| Veröffentlicht in: | Lipids 1967-01, Vol.2 (1), p.72-75 |
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| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
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| Zusammenfassung: | A chromatographic method, using columns packed with Celite‐Silica Gel G impregnated with silver nitrate, has been developed for the separation of cholesterol from desmosterol (24‐dehydrocholesterol).
The system employed allows a complete separation of the two sterols as acetates, as determined by gas‐liquid chromatography and thin‐layer chromatography.
The recovery of pure cholesterol and desmosterol is quantitative. |
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| ISSN: | 0024-4201 1558-9307 |
| DOI: | 10.1007/BF02532004 |