Cryopreservation of Papaver somniferum cell suspension cultures

Abstract The effect of culture age and various cooling rates on the survival and regrowth of cryopreserved cell cultures of PAPAVER SOMNIFERUM L. is described. Survival and regrowth following cryopreservation, in liquid nitrogen, occurred only with 4 and 5 day old cultures. No regrowth was observed...

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Veröffentlicht in:Planta medica 1991-02, Vol.57 (1), p.53-55
Hauptverfasser: Friesen, L.J, Kartha, K.K, Leung, N.L, Englund, P, Giles, K.L, Park, J, Songstad, D.D
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container_end_page 55
container_issue 1
container_start_page 53
container_title Planta medica
container_volume 57
creator Friesen, L.J
Kartha, K.K
Leung, N.L
Englund, P
Giles, K.L
Park, J
Songstad, D.D
description Abstract The effect of culture age and various cooling rates on the survival and regrowth of cryopreserved cell cultures of PAPAVER SOMNIFERUM L. is described. Survival and regrowth following cryopreservation, in liquid nitrogen, occurred only with 4 and 5 day old cultures. No regrowth was observed in 3 or 6 day old cultures. Cooling rate had a significant effect on survival following cryopreservation with an optimum cooling rate of 0.5°C min -1 .
doi_str_mv 10.1055/s-2006-960017
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language eng
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subjects Biological and medical sciences
Biotechnology
cell suspension culture
cryopreservation
cultured cells
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Miscellaneous
Papaver somniferum
Plant cells and fungal cells
title Cryopreservation of Papaver somniferum cell suspension cultures
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