Cryopreservation of Papaver somniferum cell suspension cultures
Abstract The effect of culture age and various cooling rates on the survival and regrowth of cryopreserved cell cultures of PAPAVER SOMNIFERUM L. is described. Survival and regrowth following cryopreservation, in liquid nitrogen, occurred only with 4 and 5 day old cultures. No regrowth was observed...
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Veröffentlicht in: | Planta medica 1991-02, Vol.57 (1), p.53-55 |
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creator | Friesen, L.J Kartha, K.K Leung, N.L Englund, P Giles, K.L Park, J Songstad, D.D |
description | Abstract
The effect of culture age and various cooling rates on the survival and regrowth of cryopreserved cell cultures of PAPAVER SOMNIFERUM L. is described. Survival and regrowth following cryopreservation, in liquid nitrogen, occurred only with 4 and 5 day old cultures. No regrowth was observed in 3 or 6 day old cultures. Cooling rate had a significant effect on survival following cryopreservation with an optimum cooling rate of 0.5°C min
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The effect of culture age and various cooling rates on the survival and regrowth of cryopreserved cell cultures of PAPAVER SOMNIFERUM L. is described. Survival and regrowth following cryopreservation, in liquid nitrogen, occurred only with 4 and 5 day old cultures. No regrowth was observed in 3 or 6 day old cultures. Cooling rate had a significant effect on survival following cryopreservation with an optimum cooling rate of 0.5°C min
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The effect of culture age and various cooling rates on the survival and regrowth of cryopreserved cell cultures of PAPAVER SOMNIFERUM L. is described. Survival and regrowth following cryopreservation, in liquid nitrogen, occurred only with 4 and 5 day old cultures. No regrowth was observed in 3 or 6 day old cultures. Cooling rate had a significant effect on survival following cryopreservation with an optimum cooling rate of 0.5°C min
-1
.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>cell suspension culture</subject><subject>cryopreservation</subject><subject>cultured cells</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. Technologies</subject><subject>Miscellaneous</subject><subject>Papaver somniferum</subject><subject>Plant cells and fungal cells</subject><issn>0032-0943</issn><issn>1439-0221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNp10Etr3TAQBWARWpLbNMtuE29KN3U7I8mvVSiXviDQQpO10JVHjYNtORo7kH9fGV_IqqtZ6NOZ4QjxDuETQlF85lwClHlTAmB1InaoVZODlPhK7ACUzKHR6ky8YX5IQjcAp-IMKylLlLgT1_v4HKZITPHJzl0Ys-Cz33ayTxQzDsPYeYrLkDnq-4wXnmjkVbmln5f07a147W3PdHGc5-Lu29fb_Y_85tf3n_svN7nTWM056VoeykNFVLXpqrJEakG3tWsd-EYVTWFtheDR161HPGhHUiqtfC1l1apWnYsPW-4Uw-NCPJuh4_UoO1JY2FRKo66bWiWZb9LFwBzJmyl2g43PBsGslRk2a2Vmqyz5y2PychiofdHHjhJ4fwSWne19tKPr-MU1RV1DsQZ93Nx839FA5iEscUyd_Hfv1ca9Dcb-jSny7o8EVOmxBKlL9Q9DaYqJ</recordid><startdate>19910201</startdate><enddate>19910201</enddate><creator>Friesen, L.J</creator><creator>Kartha, K.K</creator><creator>Leung, N.L</creator><creator>Englund, P</creator><creator>Giles, K.L</creator><creator>Park, J</creator><creator>Songstad, D.D</creator><general>Thieme</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19910201</creationdate><title>Cryopreservation of Papaver somniferum cell suspension cultures</title><author>Friesen, L.J ; Kartha, K.K ; Leung, N.L ; Englund, P ; Giles, K.L ; Park, J ; Songstad, D.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-e482b6b7ee7d003661ed04d8cdc0f93595aa710f1f8df11b4ce22343f8227d3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>cell suspension culture</topic><topic>cryopreservation</topic><topic>cultured cells</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Methods. Procedures. Technologies</topic><topic>Miscellaneous</topic><topic>Papaver somniferum</topic><topic>Plant cells and fungal cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Friesen, L.J</creatorcontrib><creatorcontrib>Kartha, K.K</creatorcontrib><creatorcontrib>Leung, N.L</creatorcontrib><creatorcontrib>Englund, P</creatorcontrib><creatorcontrib>Giles, K.L</creatorcontrib><creatorcontrib>Park, J</creatorcontrib><creatorcontrib>Songstad, D.D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Planta medica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Friesen, L.J</au><au>Kartha, K.K</au><au>Leung, N.L</au><au>Englund, P</au><au>Giles, K.L</au><au>Park, J</au><au>Songstad, D.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryopreservation of Papaver somniferum cell suspension cultures</atitle><jtitle>Planta medica</jtitle><addtitle>Planta Med</addtitle><date>1991-02-01</date><risdate>1991</risdate><volume>57</volume><issue>1</issue><spage>53</spage><epage>55</epage><pages>53-55</pages><issn>0032-0943</issn><eissn>1439-0221</eissn><coden>PLMEAA</coden><abstract>Abstract
The effect of culture age and various cooling rates on the survival and regrowth of cryopreserved cell cultures of PAPAVER SOMNIFERUM L. is described. Survival and regrowth following cryopreservation, in liquid nitrogen, occurred only with 4 and 5 day old cultures. No regrowth was observed in 3 or 6 day old cultures. Cooling rate had a significant effect on survival following cryopreservation with an optimum cooling rate of 0.5°C min
-1
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subjects | Biological and medical sciences Biotechnology cell suspension culture cryopreservation cultured cells Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Miscellaneous Papaver somniferum Plant cells and fungal cells |
title | Cryopreservation of Papaver somniferum cell suspension cultures |
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