Cryopreservation of Papaver somniferum cell suspension cultures

Abstract The effect of culture age and various cooling rates on the survival and regrowth of cryopreserved cell cultures of PAPAVER SOMNIFERUM L. is described. Survival and regrowth following cryopreservation, in liquid nitrogen, occurred only with 4 and 5 day old cultures. No regrowth was observed...

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Veröffentlicht in:	Planta medica 1991-02, Vol.57 (1), p.53-55
Hauptverfasser:	Friesen, L.J, Kartha, K.K, Leung, N.L, Englund, P, Giles, K.L, Park, J, Songstad, D.D
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology cell suspension culture cryopreservation cultured cells Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Miscellaneous Papaver somniferum Plant cells and fungal cells
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Zusammenfassung:	Abstract The effect of culture age and various cooling rates on the survival and regrowth of cryopreserved cell cultures of PAPAVER SOMNIFERUM L. is described. Survival and regrowth following cryopreservation, in liquid nitrogen, occurred only with 4 and 5 day old cultures. No regrowth was observed in 3 or 6 day old cultures. Cooling rate had a significant effect on survival following cryopreservation with an optimum cooling rate of 0.5°C min -1 .
ISSN:	0032-0943 1439-0221
DOI:	10.1055/s-2006-960017