CTLA4 exon 1 and promoter polymorphisms in patients with multiple sclerosis

Objective – The polymorphisms of exon 1 (+49 A/G) and promoter regions (−1722 T/C, −1661 A/G and −318 C/T)of cytotoxic T lymphocyte antigen 4 (CTLA4) and also haplotypes constructed from mentioned loci were investigated amongst 153 Iranian patients with definite multiple sclerosis (MS) and 190 healthy controls. Methods – The polymorphisms were genotyped by PCR‐restriction fragment length polymorphisms and PCR‐amplification refractory mutation system. The 4‐locus haplotypes were estimated by Arlequin software (University of Berne, Berne, Switzerland). Results – Preliminary results showed significant increase of +49 G allele and −1661 AG genotype, as well as TGCA haplotype among patients than controls (P