Monitoring of C0 and C2 Blood Concentrations of Cyclosporine on the Roche Hitachi 902 Analyzer

Abstract This work provided parameters to perform the widely used enzyme multiplied immunoassay technique (Emit) cyclosporine assay on a Hitachi 902 analyzer. Instrument settings were optimized to arrive at assay characteristics regarding precision, linearity, and lower limit of quantitation among 1...

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Veröffentlicht in:	Transplantation proceedings 2009-11, Vol.41 (9), p.3713-3719
Hauptverfasser:	Yargui, L, Berhoune, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Autoanalysis - instrumentation Autoanalysis - methods Cyclosporine - blood Cyclosporine - therapeutic use Humans Immunosuppressive Agents - blood Immunosuppressive Agents - therapeutic use Kidney Transplantation - immunology Kinetics Monitoring, Physiologic - methods Reproducibility of Results Surgery
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Zusammenfassung:	Abstract This work provided parameters to perform the widely used enzyme multiplied immunoassay technique (Emit) cyclosporine assay on a Hitachi 902 analyzer. Instrument settings were optimized to arrive at assay characteristics regarding precision, linearity, and lower limit of quantitation among 105 samples from renal transplant recipients receiving cyclosporine. We compared successive results with Emit on a Cobas Integra 400 analyzer with HPLC-UV. We validated a quantitation range of 20–500 ng/mL. For all parameters tested total imprecision
ISSN:	0041-1345 1873-2623
DOI:	10.1016/j.transproceed.2009.06.204