Nucleolar disruption impairs Kaposi’s sarcoma-associated herpesvirus ORF57-mediated nuclear export of intronless viral mRNAs

Kaposi’s sarcoma-associated herpesvirus (KSHV) ORF57 plays a pivotal role in mediating the nuclear export of KSHV intronless transcripts. We demonstrate that ORF57 localises and dynamically trafficks through the nucleolus. To assess if nucleolar shuttling of ORF57 is important for the export of intr...

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Veröffentlicht in:	FEBS letters 2009-11, Vol.583 (22), p.3549-3556
Hauptverfasser:	Boyne, James R., Whitehouse, Adrian
Format:	Artikel
Sprache:	eng
Schlagworte:	Active Transport, Cell Nucleus Blotting, Western Cell Line Cell Nucleolus - metabolism Cell Nucleolus - virology Fluorescence Recovery After Photobleaching Fluorescent Antibody Technique, Indirect Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism HeLa Cells Herpesvirus Herpesvirus 8, Human - genetics Herpesvirus 8, Human - metabolism Human herpesvirus 8 Humans Introns Kaposi's sarcoma-associated herpesvirus mRNA Nuclear export Nucleolus Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism RNA Transport RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Viral - genetics RNA, Viral - metabolism Transfection Viral Proteins - genetics Viral Proteins - metabolism
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Zusammenfassung:	Kaposi’s sarcoma-associated herpesvirus (KSHV) ORF57 plays a pivotal role in mediating the nuclear export of KSHV intronless transcripts. We demonstrate that ORF57 localises and dynamically trafficks through the nucleolus. To assess if nucleolar shuttling of ORF57 is important for the export of intronless viral mRNA, the ability of ORF57 to export mRNA was assessed in cells where the nucleolus was disrupted using Actinomycin D or DRB. We show that rapid disorganisation of the nucleolus was accompanied by a reduction in intronless virus mRNA export, suggesting that an intact nucleolus is essential for efficient KSHV ORF57-mediated intronless mRNA export. MINT-7291377: Aly (uniprotkb:Q86V81) physically interacts (MI:0915) with ORF57 (uniprotkb:Q2HR75) by pull down (MI:0096) MINT-7291330, MINT-7291320: Nucleolin (uniprotkb:P19338) and ORF57 (uniprotkb:Q2HR75) colocalise (MI:0403) by fluorescence microscopy (MI:0416) MINT-7291715: ORF57 (uniprotkb:Q2HR75) and Aly (uniprotkb:Q86V81) colocalise (MI:0403) by fluorescence microscopy (MI:0416)
ISSN:	0014-5793 1873-3468
DOI:	10.1016/j.febslet.2009.10.040