Streptomyces xinghaiensis sp. nov., isolated from marine sediment

1 Department of Bioscience and Bioengineering, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China 2 The Key Laboratory for Microbial Resources of the Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China 3 Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China 4 Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China 5 DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, 7b Inhoffenstraße, D-38124 Braunschweig, Germany 6 Department of Biological Science, Myongji University, Yongin 449-728, Republic of Korea Correspondence Feng-Wu Bai fwbai{at}dlut.edu.cn A novel actinomycete, strain S187 T , was isolated from a marine sediment sample collected from Xinghai Bay, Dalian, China. Growth occurred on ISP medium 2 containing 0–9 % NaCl and at pH 6.0–9.0 and 10–45 °C. The cell wall of strain S187 T contained the isomer LL -diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H 6 ) (40.8 %), MK-9(H 8 ) (38.2 %) and MK-9(H 2 ) (8.8 %). The major fatty acids were iso-C 16 : 0 (29.6 %), anteiso-C 15 : 0 (14.0 %) and anteiso-C 17 : 0 (11.6 %). Cells contained phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannosides and one unknown phospholipid. The G + C content of the genomic DNA was 72.01 mol%. The 16S rRNA gene sequence of the isolate had similarities of 98.1 and 97.5 % with those of Streptomyces flavofuscus NRRL B-8036 T (=DSM 41426 T ) and Streptomyces albiaxialis DSM 41799 T , respectively, showing that the novel strain should be assigned to the genus Streptomyces . DNA–DNA hybridizations with the two above-mentioned Streptomyces species showed 31.4 and 46.9 % relatedness, respectively. Moreover, the three strains differed in some physiological and biochemical properties. Thus, on the basis of phenotypic and genotypic analyses, it is proposed that strain S187 T represents a novel species of the genus Streptomyces , for which the name Streptomyces xinghaiensis sp. nov. is proposed; the type strain is S187 T (=NRRL B-24674 T =CCTCC AA 208049 T =KCTC 19546 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain S187 T is EF577247 . A