Interferon regulatory factor-2 induces megakaryopoiesis in mouse bone marrow hematopoietic cells

Megakaryopoiesis is associated with inflammatory reactions. To investigate the role of interferon regulatory factors (IRFs) in inflammation-associated megakaryopoiesis, mouse bone marrow hematopoietic stem cells (HSCs) were analyzed. IFN-γ treatment induced IRF-2 expression as well as the expression...

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Veröffentlicht in:	FEBS letters 2009-11, Vol.583 (21), p.3493-3500
Hauptverfasser:	Masumi, Atsuko, Hamaguchi, Isao, Kuramitsu, Madoka, Mizukami, Takuo, Takizawa, Kazuya, Momose, Haruka, Naito, Seishiro, Yamaguchi, Kazunari
Format:	Artikel
Sprache:	eng
Schlagworte:	5-fluorouracil 5-FU Animals Bone Marrow Cell Differentiation - drug effects Cell Line CFU-GM CFU-granulocyte/macrophage CFU-Mk colony forming unit-megakaryocyte EGFP enhanced green fluorescent protein Gene regulation hematopoietic stem cell Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - metabolism HSC Humans Inflammation Inflammation - metabolism interferon regulatory factor Interferon Regulatory Factor-2 - genetics Interferon Regulatory Factor-2 - metabolism Interferon-gamma - pharmacology interferon-stimulated response element IRF IRF-2 ISRE Megakaryocytes - cytology Megakaryocytes - metabolism Megakaryopoiesis Mice Mice, Inbred C57BL MN cells mononuclear cells Platelet Membrane Glycoprotein IIb - genetics Platelet Membrane Glycoprotein IIb - metabolism Promoter Regions, Genetic - genetics Rats Stem cell thrombopoietin TPO Transcriptional Activation - drug effects
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Zusammenfassung:	Megakaryopoiesis is associated with inflammatory reactions. To investigate the role of interferon regulatory factors (IRFs) in inflammation-associated megakaryopoiesis, mouse bone marrow hematopoietic stem cells (HSCs) were analyzed. IFN-γ treatment induced IRF-2 expression as well as the expression of CD41 and IRF-1 in HSCs. An in vitro clonogenic assay showed that IRF-2- but not IRF-1-overexpressing cells increased the number of megakaryocytic colonies. IRF-2 transfection up-regulated CD41 promoter activity in hematopoietic cell lines. The number of CD41-positive bone marrow cells increased in mice injected with IRF-2-expressing bone marrow cells. These findings suggest that IRF-2 plays an important role in megakaryopoiesis in inflammatory states.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/j.febslet.2009.10.006