A chemical platform for improved induction of human iPSCs

The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation fr...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nature methods 2009-11, Vol.6 (11), p.805-808
Hauptverfasser:	Ding, Sheng, Lin, Tongxiang, Ambasudhan, Rajesh, Yuan, Xu, Li, Wenlin, Hilcove, Simon, Abujarour, Ramzey, Lin, Xiangyi, Hahm, Heung Sik, Hao, Ergeng, Hayek, Alberto
Format:	Artikel
Sprache:	eng
Schlagworte:	Benzamides - pharmacology Biochemistry Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical engineering Biomedical Engineering/Biotechnology brief-communication Cell Differentiation - genetics Dioxoles - pharmacology Diphenylamine - analogs & derivatives Diphenylamine - pharmacology EDTA Efficiency Fibroblasts - physiology Humans Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - physiology Kinetics Life Sciences MAP Kinase Kinase 1 - antagonists & inhibitors Proteomics Pyrimidines - pharmacology Receptors, Transforming Growth Factor beta - antagonists & inhibitors Research methodology Stem cells Thiazoles - pharmacology Transduction, Genetic
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells.
ISSN:	1548-7091 1548-7105
DOI:	10.1038/nmeth.1393