In vivo evaluation of potential nematicidal properties of ethanol extract of Canthium mannii (Rubiaceae) on Heligmosomoides polygyrus parasite of rodents
The nematicidal activity of ethanol extract (ETE) of Canthium mannii (Rubiaceae) was assessed in vivo compared to that of Mebendazole on the adult of Heligmosomoides polygyrus parasite of rodents. One hundred and five Swiss white mice of two sexes aged 5–6 weeks old, and weighing between 20 and 25 g...
Gespeichert in:
Veröffentlicht in: | Veterinary parasitology 2009-12, Vol.166 (1), p.103-107 |
---|---|
Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The nematicidal activity of ethanol extract (ETE) of
Canthium mannii (Rubiaceae) was assessed
in vivo compared to that of Mebendazole on the adult of
Heligmosomoides polygyrus parasite of rodents. One hundred and five Swiss white mice of two sexes aged 5–6 weeks old, and weighing between 20 and 25
g were orally infected with a 0.8
ml of a dose of 104–120, 1-week-old
H. polygyrus infective larvae (L
3). After the pre-patent period (9–11 days), infected animals were randomly divided into 7 groups of 15 animals each. The nematicidal efficacy of the ETE was monitored through faecal egg count reduction (FECR) and total worm count reduction (TWCR). Five doses (350, 700, 1400, 2800 and 5600
mg
kg
−1 body weight) for ETE and 22
mg
kg
−1 for Mebendazole were studied using a bioassay. Mebendazole and 3% dimethylsulfoxide (DMSO) were included in the assays as reference drug and placebo, respectively. Each host received according to its weight for 7 days a daily dose of 0.7
ml of the product. The ETE for all the doses tested except the dose rate 350
mg
kg
−1
bwt was active
in vivo on the adult of
H. polygyrus and reduced significantly (
p
<
0.05) the FEC and the TWC of the nematode. The dose rate 5600
mg
kg
−1
bwt showed the highest nematicidal activity of 75.0% FECR and 83.6% TWCR 7 days post-treatment. These results supported the possible use of medicinal plants in the control of gastro-intestinal helminthiasis. |
---|---|
ISSN: | 0304-4017 1873-2550 |
DOI: | 10.1016/j.vetpar.2009.07.048 |