Isolation and characterization of a novel short-chain alcohol dehydrogenase gene from Panax ginseng

The cDNA of alcohol dehydrogenase (PgADH) was isolated and characterized from the leaf of Panax ginseng. The cDNA had an open reading frame of 801 bp and a deduced amino acid sequence of 266 residues. The calculated molecular mass of the mature protein is approximately 29 kDa with a predicated isoel...

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Veröffentlicht in:	BMB reports 2009-10, Vol.42 (10), p.673-678
Hauptverfasser:	Kim, Y.J., Kyung Hee University, Suwon, Republic of Korea, Shim, J.S., Kyung Hee University, Suwon, Republic of Korea, Lee, J.H., Kyung Hee University, Suwon, Republic of Korea, Jung, D.Y., Kyung Hee University, Suwon, Republic of Korea, Sun, H., Kyung Hee University, Suwon, Republic of Korea, In, J.G., Kyung Hee University, Suwon, Republic of Korea, Yang, D.C., Kyung Hee University, Suwon, Republic of Korea
Format:	Artikel
Sprache:	eng
Schlagworte:	Alcohol Dehydrogenase - chemistry Alcohol Dehydrogenase - genetics Alcohol Dehydrogenase - isolation & purification Amino Acid Sequence Blotting, Southern DNA, Plant - metabolism ESTRES EXPRESION GENICA EXPRESSION DES GENES GENE EXPRESSION Gene Expression Regulation, Plant Molecular Sequence Data Nucleic Acid Hybridization Panax - enzymology Panax - genetics PANAX PSEUDOGINSENG Protein Structure, Secondary RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Alignment Sequence Analysis, Protein Sequence Homology, Amino Acid Short chain-alcohol dehydrogenase STRESS Stress, Physiological Time Factors
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Zusammenfassung:	The cDNA of alcohol dehydrogenase (PgADH) was isolated and characterized from the leaf of Panax ginseng. The cDNA had an open reading frame of 801 bp and a deduced amino acid sequence of 266 residues. The calculated molecular mass of the mature protein is approximately 29 kDa with a predicated isoelectric point of 6.84. Homology analysis revealed that the deduced amino acid of PgADH shares a high degree of homology with the short-chain ADH proteins of other plants. Genomic DNA hybridization analysis indicated that PgADH represents a multi-gene family. The expression of PgADH under various environmental stresses was analyzed at different time points using real-time PCR. ABA, SA and especially JA (80-fold) significantly induced PgADH expression within 24 h of treatment. The positive responses of PgADH to abiotic stimuli suggest that ginseng ADH may protect against hormone-related environmental stresses.
ISSN:	1976-6696
DOI:	10.5483/BMBRep.2009.42.10.673